| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59390-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97912-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97913-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72517 | |||
![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0004.jpg "Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.")
![Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397-A mutation but not the S22-A mutation, while NBP1-44073 antibody recognizes the pS22-A mutation and not the pS397-A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser22]-Antibody-Western-Blot-NBP1-44072-img0001.jpg)
![Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22 [p Ser397] Antibody-Western Blot-NBP1-44073-img0004.jpg "Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.")
![Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Western Blot: ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397->A mutation but not the S22->A mutation, while NBP1-44073 antibody recognizes the pS22->A mutation and not the pS397->A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.](http://images.novusbio.com/fullsize/ARHGAP22-[p-Ser397]-Antibody-Western-Blot-NBP1-44073-img0001.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0004.jpg "Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Immunocytochemistry-Immunofluorescence-NBP2-59390-img0002.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0006.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis in A431 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0001.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0005.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Western Blot: ARHGAP22 Antibody [NBP2-39028] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/ARHGAP22-Antibody-Western-Blot-NBP2-39028-img0002.jpg "Western Blot: ARHGAP22 Antibody [NBP2-39028] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunohistochemistry: ARHGAP22 Antibody [NBP2-39028] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/ARHGAP22-Antibody-Immunohistochemistry-NBP2-39028-img0001.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] - Analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-10Y4M10-Western-Blot-NBP3-16567-img0001.jpg "Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] - Analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] - Western blot analysis of various lysates using PEDFR/PNPLA2/ATGL Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16567_rabbit-pedfr-pnpla2-atgl-mab-10y4m10-52202511392282.jpg)
![Immunohistochemistry-Paraffin: ARHGAP22 Antibody [NBP2-97912] - Immunochemical staining of human ARHGAP22 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ARHGAP22-Antibody-Immunohistochemistry-Paraffin-NBP2-97912-img0002.jpg "Immunohistochemistry-Paraffin: ARHGAP22 Antibody [NBP2-97912] - Immunochemical staining of human ARHGAP22 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: ARHGAP22 Antibody [NBP2-97912] - Immunofluorescence staining of ARHGAP22 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97912 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97912_rabbit-polyclonal-arhgap22-antibody-23520251129344.png)
![Immunohistochemistry-Paraffin: ARHGAP22 Antibody [NBP2-97913] - Immunochemical staining of human ARHGAP22 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ARHGAP22-Antibody-Immunohistochemistry-Paraffin-NBP2-97913-img0002.jpg "Immunohistochemistry-Paraffin: ARHGAP22 Antibody [NBP2-97913] - Immunochemical staining of human ARHGAP22 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: ARHGAP22 Antibody [NBP2-97913] - Immunofluorescence staining of ARHGAP22 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97913 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97913_rabbit-polyclonal-arhgap22-antibody-23520251154562.png)
![Western Blot: Lnx1 Antibody (1H9) [NBP2-45794] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lnx1.](http://images.novusbio.com/fullsize/Lnx1-Antibody-1H9-Western-Blot-NBP2-45794-img0001.jpg "Western Blot: Lnx1 Antibody (1H9) [NBP2-45794] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lnx1.")
![Western Blot: Lnx1 Antibody [NBP1-80518] - Host: Rabbit. Target Name: LNX1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Lnx1-Antibody-Western-Blot-NBP1-80518-img0004.jpg "Western Blot: Lnx1 Antibody [NBP1-80518] - Host: Rabbit. Target Name: LNX1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1ug/ml")
![Western Blot: Lnx1 Antibody [NBP1-80518] - Transfected 293T cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/Lnx1-Antibody-Western-Blot-NBP1-80518-img0003.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (S08-3F5) [NBP3-19942] - Western blot detection of PEDFR/PNPLA2/ATGL in K562 cell lysates using NBP3-19942 (1:500 diluted). Predicted band size: 55kDa. Observed band size: 55kDa.](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-S08-3F5-Western-Blot-NBP3-19942-img0001.jpg "Western Blot: PEDFR/PNPLA2/ATGL Antibody (S08-3F5) [NBP3-19942] - Western blot detection of PEDFR/PNPLA2/ATGL in K562 cell lysates using NBP3-19942 (1:500 diluted). Predicted band size: 55kDa. Observed band size: 55kDa.")
