| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88191-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89969-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54426-100ug | |||
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90125-img0015.jpg "Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0014.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0013.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0011.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human lymphoid tissues shows no positivity in non-germinal or germinal center cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0012.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211] - PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60 kDa TH phosphorylated at Ser31.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser31]-Antibody-Western-Blot-NB300-211-img0001.jpg "Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211] - PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60 kDa TH phosphorylated at Ser31.")
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ADK-Antibody-Western-Blot-NBP2-15291-img0016.jpg "Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15291-img0005.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse liver. ADK stained by Adenosine kinase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ADK-Antibody-Immunohistochemistry-Paraffin-NBP2-15291-img0014.jpg)
![Western Blot: ADK Antibody [NBP2-15291] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADK antibody (NBP2-15291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024202191.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-9102024205663.jpg)
![Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-91020242024612.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ADK Antibody [NBP2-15291] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15291_rabbit-polyclonal-adk-antibody-161020241940573.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0015.jpg "Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta stained by GSK3 beta antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0013.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - H661 xenograft, using GSK3 beta antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0009.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody [NBP1-31353] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3].](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Rat brain. GSK3 beta antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0007.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0005.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0001.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0007.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0004.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0009.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0011.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human rectum shows strong cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis using Anti-HSPA5 antibody NBP1-89969 (A) shows similar pattern to independent antibody NBP1-89968 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0014.jpg)
![HO-1/HMOX1/HSP32 Antibody (3L2U4) [NBP3-15640] - Analysis of extracts of HepG2 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-3l2u4-western-blot-45202375028..jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rabbit HO-1/HMOX1/HSP32 mAb (7H7W10) [NBP3-15640] - Analysis of extracts from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockout (KO) HeLa cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-3l2u4-knockout-validated-45202375233.jpg)
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-52202517363729.jpg)
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-5220251733556.jpg)
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-52202517352752.jpg)
![Immunocytochemistry/ Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (7H7W10) [NBP3-15640] - Immunofluorescence analysis of paraffin-embedded human spleen using [KD Validated] HO-1/HMOX1/HSP32 Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15640_rabbit-ho-1-hmox1-hsp32-mab-7h7w10-52202517303122.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0007.jpg "Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on mouse liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunohistochemistry-Paraffin-NBP2-67572-img0003.jpg)
![Western Blot: S100B Antibody (SC57-02) [NBP2-67572] - Analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Western-Blot-NBP2-67572-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (SC57-02) [NBP2-67572] - Staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100B-Antibody-SC57-02-Immunocytochemistry-Immunofluorescence-NBP2-67572-img0002.jpg)
![Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Western-Blot-NBP2-53188-img0003.jpg "Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).")
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1706R) [NBP2-53188] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Monoclonal Antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Immunohistochemistry-Paraffin-NBP2-53188-img0001.jpg)
![Protein Array: S100B Antibody (S100B/1706R) [NBP2-53188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B Antibody (S100B/1706R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Protein-Array-NBP2-53188-img0002.jpg)