| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71526 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38537-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99690-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26318-50ul | |||
![ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Samples were spiked with high concentrations of Mouse FABP2/I-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82214_mouse-fabp2-i-fabp-elisa-kit-colorimetric-13220241681478.jpg "ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Samples were spiked with high concentrations of Mouse FABP2/I-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82214_mouse-fabp2-i-fabp-elisa-kit-colorimetric-4320249115662.jpg)
![Western Blot: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4 (1:2000).](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-4F12-Western-Blot-NBP2-46221-img0004.jpg "Western Blot: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4 (1:2000).")
![Immunohistochemistry-Paraffin: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-46221-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183329.jpg "Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183323.jpg)
![Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38537_rabbit-mrp2-pab-101220241136085.jpg "Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38537_rabbit-mrp2-pab-1012202411381275.jpg)
![Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Western-Blot-NBP2-71526-img0001.jpg "Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4")
![Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71526-img0002.jpg)
![Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NBP2-99690-img0007.jpg "Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: SR-BI Antibody [NBP2-99690] - SR-BI was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-SR-BI rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SR-BI rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunoprecipitation-NBP2-99690-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NBP2-99690] - Immunofluorescence staining of SR-BI in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99690 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99690_rabbit-polyclonal-sr-bi-antibody-23520251449239.png)
![Immunohistochemistry: Glut2 Antibody (37E1) [NBP3-26318] - Image of Glut2 Antibody (37E1) diluted at 1:50 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.20% DAB.](http://images.novusbio.com/fullsize/nbp3-26318_rabbit-glut2-mab-37e1-262202415355721.jpg "Immunohistochemistry: Glut2 Antibody (37E1) [NBP3-26318] - Image of Glut2 Antibody (37E1) diluted at 1:50 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.20% DAB.")
![Immunocytochemistry/Immunofluorescence: Glut2 Antibody (37E1) [NBP3-26318] - Staining of HepG2 with Glut2 Antibody (37E1) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26318_rabbit-glut2-mab-37e1-28220241323616.jpg)
![Flow Cytometry: Glut2 Antibody (37E1) [NBP3-26318] - Overlay Peak curve showing HepG2 cells stained with Glut2 Antibody (37E1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26318_rabbit-glut2-mab-37e1-282202414312757.jpg)