| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49485-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43847 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89061-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76675-0.025mg | |||
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249342724.jpg "Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249422658.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413360.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249383187.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249362716.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413379.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249403843.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP1-84687-img0005.jpg "Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0020.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0012.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0013.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP1-84687 (A) shows similar protein distribution across tissues to independent antibody NBP2-38622 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP1-84687 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0018.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human pancreas shows moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0019.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0021.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NBP2-49485-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49485-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0006.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0007.jpg)
![Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Western-Blot-NBP2-61620-img0006.jpg "Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620] - Staining of MCF7 cells using the Anti-COX4I1 monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunocytochemistry-Immunofluorescence-NBP2-61620-img0007.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human skin shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-Paraffin-NBP2-61620-img0005.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0002.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0003.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0004.jpg)
![Western Blot: FHIT Antibody [NBP1-89061] - Analysis in control (vector only transfected HEK293T lysate) and FHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FHIT-Antibody-Western-Blot-NBP1-89061-img0006.jpg "Western Blot: FHIT Antibody [NBP1-89061] - Analysis in control (vector only transfected HEK293T lysate) and FHIT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human testis.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0010.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human kidney shows strong cytoplasmic positivity in tubules and weak staining in glomeruli.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human colon, kidney, lymph node and testis using Anti-FHIT antibody NBP1-89061 (A) shows similar protein distribution across tissues to independent antibody NBP1-89062 (B).](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0007.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human colon.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0008.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human kidney.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0009.jpg)
![Immunohistochemistry-Paraffin: FHIT Antibody [NBP1-89061] - Staining of human lymph node.](http://images.novusbio.com/fullsize/FHIT-Antibody-Immunohistochemistry-Paraffin-NBP1-89061-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-271120241918610.gif "Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining")
![Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-76675] - Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-271120242013111.gif)
![Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-76675] - Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420432366.gif)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420272431.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 ug/ml.Green: ATF6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420141974.gif)
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-76675] - Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76675_rabbit-polyclonal-atf6-antibody-2711202420115534.jpg)
![Western Blot: ATF6 Antibody [NBP1-76675] - Analysis in EL4 cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-76675-img0005.jpg)
![Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/COX-2-Antibody-Western-Blot-NB110-1948-img0001.jpg "Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.")
![Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Western-Blot-NBP3-15416-img0002.jpg "Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: COX4 Antibody (9X9I0) [NBP3-15416] - Immunohistochemistry of paraffin-embedded human colon carcinoma using COX4 Rabbit mAb (NBP3-15416) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Immunohistochemistry-Paraffin-NBP3-15416-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726031.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517223979.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human colon using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517284940.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-52202517162399.jpg)
![Immunohistochemistry: COX4 Antibody (3P8J2) [COX4] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using COX4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15416_rabbit-cox4-mab-3p8j2-5220251726025.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0004.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0005.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0003.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0006.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0013.jpg "Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0010.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0011.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-89477-img0012.jpg)
