| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38291-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38068-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-85473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90247-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99633-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00351-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99690-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97726-50ul | |||
![Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38291_rabbit-fumarylacetoacetate-hydrolase-pab-101220241214421.jpg "Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western Blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.](http://images.novusbio.com/fullsize/nbp3-38291_rabbit-fumarylacetoacetate-hydrolase-pab-3112025173180.jpg%3Ft=1701760548)
![Immunocytochemistry/ Immunofluorescence: PMM2/Phosphomannomutase 2 Antibody [NBP3-38068] - Immunofluorescence analysis of L929 cells using PMM2/Phosphomannomutase 2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38068_rabbit-pmm2-phosphomannomutase-2-pab-1012202411421084.jpg "Immunocytochemistry/ Immunofluorescence: PMM2/Phosphomannomutase 2 Antibody [NBP3-38068] - Immunofluorescence analysis of L929 cells using PMM2/Phosphomannomutase 2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-38068] - Western blot analysis of various lysates using PMM2/Phosphomannomutase 2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38068_rabbit-pmm2-phosphomannomutase-2-pab-1012202411364692.jpg)
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0006.jpg "Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.")
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0005.jpg)
![Immunohistochemistry: LAP3 Antibody (PSH01-26) [NBP3-32525] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32525) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32525) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32525_rabbit-lap3-mab-psh01-26-298202414161837.jpg "Immunohistochemistry: LAP3 Antibody (PSH01-26) [NBP3-32525] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32525) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32525) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: LAP3 Antibody (PSH01-26) [NBP3-32525] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32525) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: U-87 MG cell lysate (15 µg/Lane) Lane 4: Daudi cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Lane 6: Mouse liver tissue lysate (30 µg/Lane) Lane 7: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: Lane 1-7 (left): 1 minute; Lane 1-7 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32525_rabbit-lap3-mab-psh01-26-392024848506.jpg)
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (031) [NBP2-90247] - Staining of human SELPLG in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90247-img0003.jpg "Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (031) [NBP2-90247] - Staining of human SELPLG in human tonsil with rabbit monoclonal antibody (1:200).")
![Flow Cytometry: PSGL-1/CD162 Antibody (031) [NBP2-90247] - Analysis of Human SELPLG(CD162) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SELPLG(CD162), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-031-Flow-Cytometry-NBP2-90247-img0002.jpg)
![Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (031) [NBP2-90247] - Staining of human SELPLG in human spleen with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90247-img0001.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-99633-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-99633] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate. 4 ul anti-14-3-3 epsilon rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 epsilon rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-99633-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-99633] - Immunofluorescence staining of 14-3-3 epsilon in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99633 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99633_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511444474.png)
![Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-288202414191531.jpg "Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-2920241192318.jpg)
![Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00351 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00351, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa](http://images.novusbio.com/fullsize/nbp3-00351_rabbit-polyclonal-pmm2-phosphomannomutase-2-antibody-235202516335042.png "Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL NBP3-00351 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00351, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size: 27 kDa")
![Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - NBP3-00351 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00351_rabbit-polyclonal-pmm2-phosphomannomutase-2-antibody-235202511545675.png)
![Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NBP2-99690-img0007.jpg "Western Blot: SR-BI Antibody [NBP2-99690] - Anti-SR-BI rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: SR-BI Antibody [NBP2-99690] - SR-BI was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-SR-BI rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SR-BI rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 81 kDa](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunoprecipitation-NBP2-99690-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NBP2-99690] - Immunofluorescence staining of SR-BI in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99690 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99690_rabbit-polyclonal-sr-bi-antibody-23520251449239.png)
![Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-278202416331235.jpg "Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAP3 Antibody (PSH01-34) [NBP3-32526] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-298202414333666.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-97726-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-YWHAE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :30 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-97726-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-97726] - Immunofluorescence staining of 14-3-3 epsilon in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97726 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97726_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511545662.png)