| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14094 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12888-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42176-0.025mg | |||
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0009.jpg "Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0007.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0010.jpg)
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).")
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0001.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0002.jpg)
![Protein Array: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IGF-I R/IGF1R Antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Protein-Array-NBP3-13948-img0005.jpg)
![Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Western-Blot-NB120-3484-img0004.jpg "Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Flow-Cytometry-NB120-3484-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0002.jpg)
![Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Jurkat cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/Fumarylacetoacetate-hydrolase-Antibody-Western-Blot-NBP1-53110-img0002.jpg "Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Jurkat cell lysate, concentration 2.5 ug/ml.")
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Human Liver and Mouse FAH KO liver. Primary Dilution: 1:400](http://images.novusbio.com/fullsize/Fumarylacetoacetate-hydrolase-Antibody-Immunohistochemistry-Paraffin-NBP1-53110-img0007.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Human liver.](http://images.novusbio.com/fullsize/Fumarylacetoacetate-hydrolase-Antibody-Immunohistochemistry-Paraffin-NBP1-53110-img0001.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Fumarylacetoacetate-hydrolase-Antibody-Immunohistochemistry-Paraffin-NBP1-53110-img0003.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP1-53110] - Human liver, mouse KO tissue.](http://images.novusbio.com/fullsize/Fumarylacetoacetate-hydrolase-Antibody-Immunohistochemistry-Paraffin-NBP1-53110-img0006.jpg)
![Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-Western-Blot-NB100-1494-img0008.jpg "Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.")
![Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed U-2 OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1494-img0009.jpg)
![Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Retinol-Binding-Protein-RBP-Antibody-Flow-Cytometry-NB100-1494-img0010.jpg)
![Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Western-Blot-NBP3-16650-img0003.jpg "Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Confocal imaging of HeLa cells using CAD Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16650_rabbit-cad-mab-0o8d6-822025208484.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Western blot analysis using PPAR alpha/NR1C1 mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-5D10C3-Western-Blot-NBP2-52538-img0005.jpg "Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Western blot analysis using PPAR alpha/NR1C1 mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.")
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-5D10C3-Flow-Cytometry-NBP2-52538-img0001.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-5D10C3-Western-Blot-NBP2-52538-img0002.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-5D10C3-Western-Blot-NBP2-52538-img0003.jpg)
![ELISA: PPAR alpha/NR1C1 Antibody (5D10C3) [NBP2-52538] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-5D10C3-ELISA-NBP2-52538-img0004.jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)
![Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Western-Blot-NBP2-42176-img0004.jpg "Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)