| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05935-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57307SS | |||
| Azide and BSA Free | NBP2-80581 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48894-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77008-0.025mg | |||
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Western blot analysis of extracts of various cell lines, using FABP1/L-FABP Rabbit mAb (NBP3-15353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Western-Blot-NBP3-15353-img0003.jpg "Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Western blot analysis of extracts of various cell lines, using FABP1/L-FABP Rabbit mAb (NBP3-15353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of U-2 OS cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Immunocytochemistry-Immunofluorescence-NBP3-15353-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of NIH-3T3 cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Immunocytochemistry-Immunofluorescence-NBP3-15353-img0001.jpg)
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of various lysates using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15353_rabbit-fabp1-l-fabp-mab-8q8s10-52202517192965.jpg)
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of lysates from Hep G2 cells using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15353_rabbit-fabp1-l-fabp-mab-8q8s10-52202517284936.jpg)
![Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP3-05935-img0001.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa")
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of human PLCD1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05935-img0002.jpg)
![Immunoprecipitation: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A:0.5 mg NIH3T3 Whole Cell Lysate4 uL anti-PLCD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLCD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 86 kDaObserved band size :90 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunoprecipitation-NBP3-05935-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of PLCD1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05935 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-05935_rabbit-polyclonal-phospholipase-c-delta-1-antibody-235202511494035.png)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (4H8H8E2) [NB110-57307] - Apolipoprotein A5 antibody (1:250) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-4H8H8E2-Immunocytochemistry-Immunofluorescence-NB110-57307-img0001.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (4H8H8E2) [NB110-57307] - Apolipoprotein A5 antibody (1:250) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: DAK Antibody [NBP2-48894] - Staining of human duodenum, kidney, liver and skeletal muscle using Anti-TKFC antibody NBP2-48894 (A) shows similar protein distribution across tissues to independent antibody NBP1-84142 (B).](http://images.novusbio.com/fullsize/DAK-Antibody-Immunohistochemistry-Paraffin-NBP2-48894-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DAK Antibody [NBP2-48894] - Staining of human duodenum, kidney, liver and skeletal muscle using Anti-TKFC antibody NBP2-48894 (A) shows similar protein distribution across tissues to independent antibody NBP1-84142 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: DAK Antibody [NBP2-48894] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissue](http://images.novusbio.com/fullsize/DAK-Antibody-Western-Blot-NBP2-48894-img0002.jpg)
![Immunohistochemistry-Paraffin: DAK Antibody [NBP2-48894] - Staining of human liver.](http://images.novusbio.com/fullsize/DAK-Antibody-Immunohistochemistry-Paraffin-NBP2-48894-img0007.jpg)
![Immunohistochemistry: DAK Antibody [NBP2-48894] - Staining of human small intestine shows cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DAK-Antibody-Immunohistochemistry-NBP2-48894-img0001.jpg)
![Immunohistochemistry-Paraffin: DAK Antibody [NBP2-48894] - Staining of human kidney.](http://images.novusbio.com/fullsize/DAK-Antibody-Immunohistochemistry-Paraffin-NBP2-48894-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0009.jpg "Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0007.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0010.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)
![Western Blot: Phospholipase C delta 1 Antibody [NBP2-19810] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP2-19810-img0005.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP2-19810] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/ Immunofluorescence: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunocytochemistry--Immunofluorescence-NBP2-19810-img0004.jpg)
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP2-19810] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19810-img0002.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP1-77008] - Human liver tissue lysate with ApoA1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP1-77008-img0003.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP1-77008] - Human liver tissue lysate with ApoA1 antibody at 1 ug/ml.")
![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP1-77008] - Immunofluorescence of Apolipoprotein A-I/ApoA1 in mouse liver tissue with Apolipoprotein A-I/ApoA1 antibody at 20 ug/mL.Red: Apolipoprotein A-I/ApoA1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77008_chicken-polyclonal-apolipoprotein-a-i-apoa1-antibody-2711202420414563.gif)
![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP1-77008] - Immunofluorescence of Apolipoprotein A-I/ApoA1 in human liver tissue with Apolipoprotein A-I/ApoA1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77008_chicken-polyclonal-apolipoprotein-a-i-apoa1-antibody-2711202420392739.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-7Z9H0-Western-Blot-NBP3-16525-img0002.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3X3T8) [NBP3-16525] - Western blot analysis of various lysates, using PI 3-Kinase p85 alpha Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16525_rabbit-pi-3-kinase-p85-alpha-mab-3x3t8-52202511365130.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Western-Blot-NBP3-16847-img0003.jpg "Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded mouse testis using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0002.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded rat pancreas using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0001.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of various lysates using PLTP Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16847_rabbit-pltp-mab-6a1q1-52202511361237.jpg)
![Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-288202412442940.jpg "Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-298202414262627.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunocytochemistry analysis of A549 cells labeling SEC14L2 with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-29202410234211.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0007.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0008.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Analysis of PIK3R1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Western-Blot-H00005295-M03-img0009.jpg)
![Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3C11) [H00005295-M03] - Detection limit for recombinant GST tagged PIK3R1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-3C11-Sandwich-ELISA-H00005295-M03-img0006.jpg)