| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35913-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85906-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34024-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83255-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03522-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35630-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97524-50ul | |||
![Western Blot: CLPP Antibody (1W6O4) [NBP3-16156] - Western blot analysis of extracts of various cell lines, using CLPP Rabbit mAb (NBP3-16156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Western-Blot-NBP3-16156-img0004.jpg "Western Blot: CLPP Antibody (1W6O4) [NBP3-16156] - Western blot analysis of extracts of various cell lines, using CLPP Rabbit mAb (NBP3-16156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156] - Immunofluorescence analysis of NIH-3T3 cells using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunocytochemistry-Immunofluorescence-NBP3-16156-img0003.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156] - Immunohistochemistry of paraffin-embedded human colon using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunohistochemistry-Paraffin-NBP3-16156-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156] - Immunohistochemistry of paraffin-embedded rat kidney using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunohistochemistry-Paraffin-NBP3-16156-img0001.jpg)
![Western Blot: Citidine Deaminase Antibody [NBP3-35913] - Western blot analysis of lysates from Mouse kidney, using Citidine Deaminase Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35913_rabbit-citidine-deaminase-pab-1012202411433447.jpg "Western Blot: Citidine Deaminase Antibody [NBP3-35913] - Western blot analysis of lysates from Mouse kidney, using Citidine Deaminase Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Citidine Deaminase Antibody [NBP3-35913] - Immunofluorescence analysis of PC-12 cells using Citidine Deaminase Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35913_rabbit-citidine-deaminase-pab-1012202411421029.jpg)
![Immunocytochemistry/ Immunofluorescence: Citidine Deaminase Antibody [NBP3-35913] - Immunofluorescence analysis of HeLa cells using Citidine Deaminase Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35913_rabbit-citidine-deaminase-pab-1012202411404051.jpg)
![Immunocytochemistry/ Immunofluorescence: Citidine Deaminase Antibody [NBP3-35913] - Immunofluorescence analysis of HepG2 cells using Citidine Deaminase Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35913_rabbit-citidine-deaminase-pab-1012202411404035.jpg)
![Immunocytochemistry/ Immunofluorescence: Citidine Deaminase Antibody [NBP3-35913] - Immunofluorescence analysis of NIH/3T3 cells using Citidine Deaminase Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35913_rabbit-citidine-deaminase-pab-1012202411364617.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)
![Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GALM-Antibody-Immunohistochemistry-Paraffin-NBP1-85906-img0011.jpg "Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/GALM-Antibody-Immunohistochemistry-Paraffin-NBP1-85906-img0006.jpg)
![Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/GALM-Antibody-Immunohistochemistry-Paraffin-NBP1-85906-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining in human kidney and skin tissues using anti-GALM antibody. Corresponding GALM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GALM-Antibody-Immunohistochemistry-Paraffin-NBP1-85906-img0008.jpg)
![Immunohistochemistry-Paraffin: GALM Antibody [NBP1-85906] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/GALM-Antibody-Immunohistochemistry-Paraffin-NBP1-85906-img0010.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human liver.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP2-34024-img0006.jpg "Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human liver.")
![Immunohistochemistry: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts, Leydig cells were strongly stained.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-NBP2-34024-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human colon, kidney, liver and pancreas using Anti-MUT antibody NBP2-34024 (A) shows similar protein distribution across tissues to independent antibody NBP1-87423 (B).](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP2-34024-img0003.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human pancreas.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP2-34024-img0004.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human colon.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP2-34024-img0005.jpg)
![Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-34024] - Staining of human kidney.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Immunohistochemistry-Paraffin-NBP2-34024-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CBS Antibody [NBP1-83255] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CBS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83255-img0011.jpg "Immunocytochemistry/Immunofluorescence: CBS Antibody [NBP1-83255] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CBS Antibody [NBP1-83255] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CBS-Antibody-Immunohistochemistry-Paraffin-NBP1-83255-img0010.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody [NBP1-83255] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CBS-Antibody-Immunohistochemistry-Paraffin-NBP1-83255-img0007.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody [NBP1-83255] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CBS-Antibody-Immunohistochemistry-Paraffin-NBP1-83255-img0008.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody [NBP1-83255] - Staining of human cerebellum shows moderate cytoplasmic positivity in a subset of cells in granular layer, as well as in neuropil of molecular layer.](http://images.novusbio.com/fullsize/CBS-Antibody-Immunohistochemistry-Paraffin-NBP1-83255-img0009.jpg)
![Western Blot: CLPP Antibody [NBP1-31011] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP1-31011-img0004.jpg "Western Blot: CLPP Antibody [NBP1-31011] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP1-31011] - Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: CLPP protein stained by CLPP antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31011-img0005.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-31011] - Paraffin-embedded H520 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-31011-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CLPP Antibody [NBP1-31011] - Wild-type (WT) and CLPP knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31011_rabbit-polyclonal-clpp-antibody-1610202419414599.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of C6 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202415161829.jpg "Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of C6 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of U-2 OS cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202414112844.jpg)
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of L929 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202415121391.jpg)
![Western Blot: ETFB Antibody [NBP3-35630] - Western blot analysis of various lysates using ETFB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202414213765.jpg)
![Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/ETFB-Antibody-Western-Blot-NBP2-97524-img0005.jpg "Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunohistochemistry-Paraffin-NBP2-97524-img0004.jpg)
![Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in cynomolgus heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunohistochemistry-Paraffin-NBP2-97524-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP2-97524] - Immunofluorescence staining of ETFB in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97524 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97524_rabbit-polyclonal-etfb-antibody-235202514492380.png)
![Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-278202415295516.jpg "Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-HADH antibody (NBP3-32416) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32416) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29820241420931.jpg)
![Immunocytochemistry/ Immunofluorescence: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunocytochemistry analysis of HeLa cells labeling HADH with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29202410334032.jpg)