| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52534-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37387-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52535-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89510-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
![Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Western-Blot-NBP3-16748-img0004.jpg "Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded mouse testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0003.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded rat testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0001.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded human colon using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0002.jpg)
![Western Blot:Tyrosine Hydroxylase Antibody (OTI3G3)[NBP3-44861]](http://images.novusbio.com/fullsize/nbp3-44861_mouse-tyrosine-hydroxylase-mab-oti3g3-western-blot-181202616171519.jpg "Western Blot:Tyrosine Hydroxylase Antibody (OTI3G3)[NBP3-44861]")
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI3G3)[NBP3-44861]](http://images.novusbio.com/fullsize/nbp3-44861_mouse-tyrosine-hydroxylase-mab-oti3g3-immunohistochemistry-18120261695310.jpg)
![Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI3G3)[NBP3-44861]](http://images.novusbio.com/fullsize/nbp3-44861_mouse-tyrosine-hydroxylase-mab-oti3g3-immunofluorescence-1812026169533.jpg)
![Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Western-Blot-NBP2-52534-img0005.jpg "Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Immunofluorescence-NBP2-52534-img0002.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-Flow-Cytometry-NBP2-52534-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-3A8B6-ELISA-NBP2-52534-img0004.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0004.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.")
![Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Flow-Cytometry-NBP2-37387-img0005.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0003.jpg)
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-ELISA-NBP2-37387-img0001.jpg)
![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0002.jpg "Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against HEK293 (1) and PLCG2 (AA: 826-985)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Immunohistochemical analysis of paraffin-embedded Hela tissues using PLC-gamma 2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Immunohistochemistry-Paraffin-NBP2-52535-img0004.jpg)
![Western Blot: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Analysis using PLCG2 mAb against human PLCG2 (AA: 826-985) recombinant protein. (Expected MW is 44.6 kDa)](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-Western-Blot-NBP2-52535-img0001.jpg)
![ELISA: PLC-gamma 2 Antibody (1E10C11) [NBP2-52535] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/PLC-gamma-2-Antibody-1E10C11-ELISA-NBP2-52535-img0003.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0006.jpg "Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.")
![Immunohistochemistry: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse NGAL antibody at dilution of 1:1000. Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-Immunohistochemistry-NBP1-05183-img0005.jpg)
![ELISA: Lipocalin-2/NGAL Antibody (29) - BSA Free [NBP1-05183] - The figure illustrates the calibration curve of a sandwich assay with NBP2-21746 as the capture antibody and NBP1-05183B as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-29-BSA-Free-ELISA-NBP1-05183-img0007.jpg)
![Western Blot: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Western blot analysis of Myeloperoxidase/MPO on HL-60 cell lysates with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55/37 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32623) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-27820241631665.jpg "Western Blot: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Western blot analysis of Myeloperoxidase/MPO on HL-60 cell lysates with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84/55/37 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32623) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32623) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-29820241492616.jpg)
![Immunocytochemistry/ Immunofluorescence: Myeloperoxidase/MPO Antibody (A1F2-R) [NBP3-32623] - Immunocytochemistry analysis of HL-60 cells labeling Myeloperoxidase/MPO with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myeloperoxidase/MPO antibody (NBP3-32623) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32623_mouse-myeloperoxidase-mpo-mab-a1f2-r-29202410484828.jpg)
![Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-515-Immunocytochemistry-Immunofluorescence-NBP2-89510-img0001.jpg "Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of human CA14 in human liver with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of hepatocyte.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-515-Immunohistochemistry-Paraffin-NBP2-89510-img0002.jpg)
![Western Blot: Rabbit Monoclonal Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - 20ug/lane human melanoma cancer cell lysates (Lane #1 SKMEL3, Lane #2 M202), blotted with Carbonic Anhydrase XIV/CA14 antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89510_rabbit-monoclonal-carbonic-anhydrase-xiv-ca14-antibody-515-western-blot-712202411490..png)
![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Western-Blot-NBP1-42461-img0002.jpg "Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.")
![SDS-Page: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - This product has a purity >95%. This protein was run on a 4-20% SDS-PAGE gel with 5 ug protein loaded.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Immunoblotting-NBP1-42461-img0001.jpg)