| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35890-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38537-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38291-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59338-25ug | |||
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183329.jpg "Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183323.jpg)
![Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK](http://images.novusbio.com/fullsize/11-beta-HSD1-Antibody-Western-Blot-NBP2-27469-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK")
![<b>Genetic Strategies Validation. </b>Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/11-beta-HSD1-Antibody-Western-Blot-NBP2-27469-img0003.jpg)
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0003.jpg "Western Blot: CYP11B2 Antibody [NBP1-56518] - Transfected HEK293-CYP11B2, Antibody Titration: 0.2-1 ug/ml")
![Western Blot: CYP11B2 Antibody [NBP1-56518] - Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug) 2. HEK293TN-GFP-hcyp11B2 (75ug) Primary Dilution: 1:100 Secondary Antibody: mouse anti-Rabbit HRP Secondary Dilution: 1:10,000 Film Exposed for: 15 minutes](http://images.novusbio.com/fullsize/CYP11B2-Antibody-Western-Blot-NBP1-56518-img0002.jpg)
![Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP3-35890] - Immunofluorescence analysis of HeLa cells using ACADM Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35890_rabbit-acadm-pab-101220241224166.jpg "Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP3-35890] - Immunofluorescence analysis of HeLa cells using ACADM Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: ACADM Antibody [NBP3-35890] - Western blot analysis of various lysates using ACADM Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35890_rabbit-acadm-pab-10122024121444.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0003.jpg "Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody (15E5) [NBP2-22565] - Analysis of ACAT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Immunocytochemistry-Immunofluorescence-NBP2-22565-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0001.jpg)
![Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38537_rabbit-mrp2-pab-101220241136085.jpg "Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: MRP2 Antibody [NBP3-38537] - Western blot analysis of various lysates using MRP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38537_rabbit-mrp2-pab-1012202411381275.jpg)
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0002.jpg "Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422] - Rat kidney using GCLC Rabbit mAb (NBP3-16422) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Immunohistochemistry-Paraffin-NBP3-16422-img0001.jpg)
![Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of various cell lines, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GCLC-Antibody-0X1H3-Western-Blot-NBP3-16422-img0003.jpg)
![Immunohistochemistry: Thyroid Peroxidase Antibody (3C4A4) [NBP3-33200] - Immunohistochemistry analysis of paraffin-embedded Rat thyroid using Thyroid Peroxidase Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33200_rabbit-thyroid-peroxidase-mab-3c4a4-101220241134338.jpg "Immunohistochemistry: Thyroid Peroxidase Antibody (3C4A4) [NBP3-33200] - Immunohistochemistry analysis of paraffin-embedded Rat thyroid using Thyroid Peroxidase Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Immunohistochemistry: Thyroid Peroxidase Antibody (3C4A4) [NBP3-33200] - Immunohistochemistry analysis of paraffin-embedded Mouse thyroid using Thyroid Peroxidase Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33200_rabbit-thyroid-peroxidase-mab-3c4a4-101220241134330.jpg)
![Immunohistochemistry: Thyroid Peroxidase Antibody (3C4A4) [NBP3-33200] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Thyroid Peroxidase Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33200_rabbit-thyroid-peroxidase-mab-3c4a4-101220241136040.jpg)
![Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38291_rabbit-fumarylacetoacetate-hydrolase-pab-101220241214421.jpg "Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP3-38291] - Western Blot analysis of various lysates using Fumarylacetoacetate hydrolase Rabbit pAb at 1:10000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.](http://images.novusbio.com/fullsize/nbp3-38291_rabbit-fumarylacetoacetate-hydrolase-pab-3112025173180.jpg%3Ft=1701760548)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Western-Blot-NBP2-59338-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.")
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody, Clone 6B8-2F2 (NBP2-59338). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody (NBP2-59338) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Immunocytochemistry-Immunofluorescence-NBP2-59338-img0005.jpg)
![Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Western-Blot-NBP2-71526-img0001.jpg "Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4")
![Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71526-img0002.jpg)