| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12868-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38440-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97071-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97895-50ul | |||
![Western Blot: PGM1 Antibody (AT84G2) [NBP3-12868] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12868 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: 293T cell lysate. Lane 2: HepG2 cell lysate. Lane 3: NIH/3T3 cell lysate. Lane 4: Jurkat cell lysate. Lane 5: HeLa cell lysate. Lane 6: U87MG cell lysate](http://images.novusbio.com/fullsize/PGM1-Antibody-AT84G2-Western-Blot-NBP3-12868-img0002.jpg "Western Blot: PGM1 Antibody (AT84G2) [NBP3-12868] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12868 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: 293T cell lysate. Lane 2: HepG2 cell lysate. Lane 3: NIH/3T3 cell lysate. Lane 4: Jurkat cell lysate. Lane 5: HeLa cell lysate. Lane 6: U87MG cell lysate")
![Immunocytochemistry/Immunofluorescence: PGM1 Antibody (AT84G2) [NBP3-12868] - HeLa cells were stained with NBP3-12868 (1:100). Alexa Fluor 488-conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/PGM1-Antibody-AT84G2-Immunocytochemistry-Immunofluorescence-NBP3-12868-img0001.jpg)
![Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.](http://images.novusbio.com/fullsize/HIF-2-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10286-img0002.jpg "Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.")
![Western Blot: CYB5R3 Antibody [NBP3-38440] - Western blot analysis of various lysates using CYB5R3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38440_rabbit-cyb5r3-pab-101220241154557.jpg "Western Blot: CYB5R3 Antibody [NBP3-38440] - Western blot analysis of various lysates using CYB5R3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Glucokinase-GCK-Antibody-4G6-Western-Blot-NBP2-37579-img0003.jpg "Western Blot: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Analysis using GCK mAb against human GCK (AA: 1-198) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/Glucokinase-GCK-Antibody-4G6-Western-Blot-NBP2-37579-img0002.jpg)
![ELISA: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Glucokinase-GCK-Antibody-4G6-ELISA-NBP2-37579-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-29202410484840.jpg "Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: 293T cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: MCF7 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-3920248485023.jpg)
![Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-97071] - Immunochemical staining of human PKLR in human muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PKLR-Antibody-Immunohistochemistry-Paraffin-NBP2-97071-img0003.jpg "Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-97071] - Immunochemical staining of human PKLR in human muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-97071] - Immunochemical staining of human PKLR in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PKLR-Antibody-Immunohistochemistry-Paraffin-NBP2-97071-img0001.jpg)
![Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-97071] - Immunochemical staining of human PKLR in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PKLR-Antibody-Immunohistochemistry-Paraffin-NBP2-97071-img0002.jpg)
![Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP2-97895] - Immunochemical staining of human ACOX1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ACOX1-Antibody-Immunohistochemistry-Paraffin-NBP2-97895-img0002.jpg "Immunohistochemistry-Paraffin: ACOX1 Antibody [NBP2-97895] - Immunochemical staining of human ACOX1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: ACOX1 Antibody [NBP2-97895] - Immunofluorescence staining of ACOX1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97895 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97895_rabbit-polyclonal-acox1-antibody-23520251664775.png)
![Western Blot: PFKL Antibody [NBP2-32212] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PFKL antibody NBP2-32212 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PFKL-Antibody-Western-Blot-NBP2-32212-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PFKL Antibody [NBP2-32212] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PFKL antibody NBP2-32212 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PFKL Antibody [NBP2-32212] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PFKL antibody NBP2-32212 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFKL, NBP2-32212 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PFKL-Antibody-Immunoprecipitation-NBP2-32212-img0002.jpg)