| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87083-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14094 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87082-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81742-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42176-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12888-0.025mg | |||
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human cerebral cortex, pancreas, prostate and rectum using Anti-CANT1 antibody NBP1-87083 (A) shows similar protein distribution across tissues to independent antibody NBP1-87082 (B).](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human cerebral cortex, pancreas, prostate and rectum using Anti-CANT1 antibody NBP1-87083 (A) shows similar protein distribution across tissues to independent antibody NBP1-87082 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining in human rectum and pancreas tissues using anti-CANT1 antibody. Corresponding CANT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0005.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human prostate.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0010.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87083] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87083-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).")
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0001.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with IGF-I R/IGF1R antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Immunohistochemistry-Paraffin-NBP3-13948-img0002.jpg)
![Protein Array: IGF-I R/IGF1R Antibody (IGF1R/4667) [NBP3-13948] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IGF-I R/IGF1R Antibody (IGF1R/4667).](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-IGF1R-4667-Protein-Array-NBP3-13948-img0005.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0009.jpg "Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0007.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0010.jpg)
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining in human rectum and pancreas tissues using anti-CANT1 antibody. Corresponding CANT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining in human rectum and pancreas tissues using anti-CANT1 antibody. Corresponding CANT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining of human cerebral cortex, pancreas, prostate and rectum using Anti-CANT1 antibody NBP1-87082 (A) shows similar protein distribution across tissues to independent antibody NBP1-87081 (B).](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining of human prostate.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0009.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0005.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium Activated Nucleotidase 1/CANT1 Antibody [NBP1-87082] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Calcium-Activated-Nucleotidase-1-CANT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87082-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-81742] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glucokinase-GCK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81742-img0005.jpg "Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-81742] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-81742] - Staining of human pancreas shows moderate cytoplasmic positivity in islet cells.](http://images.novusbio.com/fullsize/Glucokinase-GCK-Antibody-Immunohistochemistry-Paraffin-NBP1-81742-img0001.jpg)
![Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Western-Blot-NBP3-16650-img0003.jpg "Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Confocal imaging of HeLa cells using CAD Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16650_rabbit-cad-mab-0o8d6-822025208484.jpg)
![Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Western-Blot-NBP2-42176-img0004.jpg "Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)
![Western Blot: Insulin Receptor beta Antibody (C18C4) [NBP1-19192] - Lane 1: MW Marker, Lane 2: HeLa lysate, Lane 3: MCF-7 lysate, Lane 4: PC-12 lysate, Lane 5: 3T3 lysate.](http://images.novusbio.com/fullsize/Insulin-Receptor-beta-Antibody-C18C4-Western-Blot-NBP1-19192-img0005.jpg "Western Blot: Insulin Receptor beta Antibody (C18C4) [NBP1-19192] - Lane 1: MW Marker, Lane 2: HeLa lysate, Lane 3: MCF-7 lysate, Lane 4: PC-12 lysate, Lane 5: 3T3 lysate.")
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)