| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86010-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83721-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35106-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38190-20ul | |||
![Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Western-Blot-NBP1-00154-img0006.jpg "Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00154-img0007.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0005.jpg)
![Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Flow-Cytometry-NBP1-00154-img0008.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD38 Antibody [NBP1-86010] - Staining of human cell line A549 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86010-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD38 Antibody [NBP1-86010] - Staining of human cell line A549 shows localization to plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining in human tonsil and kidney tissues using NBP1-86010 antibody. Corresponding CD38 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0021.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0017.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human prostate shows strong membranous/cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0018.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human rectum shows strong membranous/cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0019.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody [NBP1-86010] - Staining of human tonsil shows strong membranous/cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD38-Antibody-Immunohistochemistry-Paraffin-NBP1-86010-img0020.jpg)
![Western Blot: MafB Antibody [NBP3-14673] - Antibody: Rabbit anti-MafB recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgGChemiluminescence with an exposure time of 3 minutes.Lower Panels: Rabbit anti-MafA recombinant monoclonalantibody and rabbit anti-Actinrecombinant monoclonal antibody.](http://images.novusbio.com/fullsize/MafB-Antibody-Western-Blot-NBP3-14673-img0002.jpg "Western Blot: MafB Antibody [NBP3-14673] - Antibody: Rabbit anti-MafB recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgGChemiluminescence with an exposure time of 3 minutes.Lower Panels: Rabbit anti-MafA recombinant monoclonalantibody and rabbit anti-Actinrecombinant monoclonal antibody.")
![Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/MafB-Antibody-Immunohistochemistry-NBP3-14673-img0005.jpg)
![Western Blot: MafB Antibody [NBP3-14673] - Whole cell lysate (50 ug) from HeLa, HEK293T, SK-N-MC, RPMI-8226, Jurkat, KG-1, K-562, and MOLT-4 cells prepared using NETN lysis buffer. Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (NBP3-14673 lot 2) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2.](http://images.novusbio.com/fullsize/MafB-Antibody-Western-Blot-NBP3-14673-img0001.jpg)
![Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MafB-Antibody-Immunohistochemistry-NBP3-14673-img0004.jpg)
![Immunoprecipitation: MafB Antibody [NBP3-14673] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SK-N-MC cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiMafB recombinant monoclonal antibody used for IP at 6 ul/mg lysate. MafB wasalso immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated MafB, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MafB-Antibody-Immunoprecipitation-NBP3-14673-img0003.jpg)
![Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0009.jpg "Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ketohexokinase Antibody [NBP1-32778] - Sample (20 ug of whole cell lysate) A: mouse Liver 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0007.jpg)
![Immunohistochemistry-Paraffin: ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0002.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded mouse kidney. Ketohexokinase antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0005.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ketohexokinase stained by Ketohexokinase antibody [N1C3] (NBP1-32778) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32778_rabbit-polyclonal-ketohexokinase-antibody-91020242021965.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Aspartate Aminotransferase Antibody [NBP2-57518] - Analysis using Anti-GOT1 antibody NBP2-57518 (A) shows similar pattern to independent antibody NBP2-55813 (B).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP2-57518-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57518-img0001.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0004.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0005.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody [NBP1-83721] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunohistochemistry-Paraffin-NBP1-83721-img0004.jpg "Immunohistochemistry-Paraffin: Kif4A Antibody [NBP1-83721] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.")
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.")
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0007.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD38 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0002.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0004.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0005.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0006.jpg)
![Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252857.jpg "Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Human stomach using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120688.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252836.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120624.jpg)
![Immunocytochemistry/ Immunofluorescence: Kif4A Antibody [NBP3-35106] - Immunofluorescence analysis of HeLa cells using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241157269.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241252813.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human colon using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241233823.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP3-38190] - Western blot analysis of various lysates using Aspartate Aminotransferase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-1012202411592816.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-10122024120650.jpg)
![Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunofluorescence analysis of U2OS cells using Aspartate Aminotransferase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241243928.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.")
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0006.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0002.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0004.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0005.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0007.jpg)
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)