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![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NB100-1940-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL.Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NB100-1940-img0003.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Western-Blot-NBP2-42614-img0001.jpg "Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (55D5) [NBP2-42614] - ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Immunocytochemistry-Immunofluorescence-NBP2-42614-img0004.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: ACAT2 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0002.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: Ramos cell lysateLane 3.: MCF7 cell lysateLane 4.: HL-60 cell lysateLane 5.: SW480 cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0003.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - Analysis of ACAT2 in PC3 cells. The cells were stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0005.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32169-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate 293T cells.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunoprecipitation-NBP2-32169-img0003.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-44228-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44228-img0001.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-7T10F5-Western-Blot-NBP3-16288-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Confocal imaging of A549 cells using AKR1C3 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16288_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-7t10f5-immunocytochemistry-immunofluorescence-186202491659..jpg)
![Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Confocal imaging of A549 cells using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16288_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-7t10f5-52202511361261.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-33556-img0006.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-33556-img0004.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-33556-img0005.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-32170-img0004.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Western blot detection of Muscle Phosphofructokinase/PFKM/PFK-1 in C6, 3T3, Hela cell lysates using NBP3-20013 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-S08-6B4-Western-Blot-NBP3-20013-img0002.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Western blot detection of Muscle Phosphofructokinase/PFKM/PFK-1 in C6, 3T3, Hela cell lysates using NBP3-20013 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Immunofluorescence of Muscle Phosphofructokinase/PFKM/PFK-1 (green) in Hela cells using NBP3-20013 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-20013_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-s08-6b4-113202515145460.jpg)
![Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Hs-8-Flow-Cytometry-NB500-456-img0001.jpg "Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.")
![Western Blot: AK7 Antibody [NBP1-32560] - Sample(30 ug whole cell lysate)A:A431 7. 5% SDS PAGE, antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/AK7-Antibody-Western-Blot-NBP1-32560-img0003.jpg "Western Blot: AK7 Antibody [NBP1-32560] - Sample(30 ug whole cell lysate)A:A431 7. 5% SDS PAGE, antibody diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: AK7 Antibody [NBP1-32560] - Analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AK7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32560-img0002.jpg)