| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90022-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90710-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11920-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89538-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99334-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89306-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89930-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90523-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90461-50ul | |||
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Western-Blot-NBP2-90710-img0003.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa")
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Immunoprecipitation-NBP2-90710-img0002.jpg)
![Immunohistochemistry: ELOVL4 Antibody [NBP3-11920] - Immunohistochemical staining of normal human testis tissue using ELOV4 antibody at 15 ug/ml.](http://images.novusbio.com/fullsize/ELOVL4-Antibody-Immunohistochemistry-NBP3-11920-img0001.jpg "Immunohistochemistry: ELOVL4 Antibody [NBP3-11920] - Immunohistochemical staining of normal human testis tissue using ELOV4 antibody at 15 ug/ml.")
![Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-278202416331284.png "Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402] - Flow cytometric analysis of Hela cells labeling GPD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32402, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-392024884666.png)
![Western Blot: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:34 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-III-CA3-Antibody-006-Western-Blot-NBP2-89538-img0001.jpg "Western Blot: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:34 kDa")
![Immunoprecipitation: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-CA3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CA3 rabbit monoclonal antibody,at 1:250 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size: 30 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-III-CA3-Antibody-006-Immunoprecipitation-NBP2-89538-img0002.jpg)
![Immunohistochemistry-Paraffin: HSD3B1 Antibody [NBP2-99334] - Immunochemical staining of human HSD3B1 in human placenta with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-99334-img0002.jpg "Immunohistochemistry-Paraffin: HSD3B1 Antibody [NBP2-99334] - Immunochemical staining of human HSD3B1 in human placenta with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: HSD3B1 Antibody [NBP2-99334] - Immunochemical staining of human HSD3B1 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-Immunohistochemistry-Paraffin-NBP2-99334-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-89306-img0001.jpg "Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Flow-Cytometry-NBP2-89306-img0002.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0002.jpg "Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human lung cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32409_mouse-grp78-hspa5-mab-a5-c9-g6-r-278202415242693.jpg "Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: LDLR Antibody (032) [NBP2-90523] - Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LDLR-Antibody-032-Flow-Cytometry-NBP2-90523-img0001.jpg "Flow Cytometry: LDLR Antibody (032) [NBP2-90523] - Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (032) [NBP2-90523] - Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90523 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG Secondary antibody(green), counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/nbp2-90523_rabbit-monoclonal-ldlr-antibody-032-235202511254411.png)
![Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Flow cytometric analysis of Rat CD59 expression on SD rat bone marrow cells. Cells were stained with purified anti-Rat CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-008-Flow-Cytometry-NBP2-90461-img0001.jpg "Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Flow cytometric analysis of Rat CD59 expression on SD rat bone marrow cells. Cells were stained with purified anti-Rat CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/ Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Immunofluorescence staining of RBP4/Retinol-Binding Protein 4 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90461 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-90461_rabbit-monoclonal-rbp4-retinol-binding-protein-4-antibody-008-235202512205528.png)