| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97726-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06252-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89374-50ul | |||
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody (OTI2C8) - Azide and BSA Free [NBP2-72652] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Methylmalonyl Coenzyme A.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-OTI2C8-Azide-and-BSA-Free-Western-Blot-NBP2-72652-img0001.jpg "Western Blot: Methylmalonyl Coenzyme A mutase Antibody (OTI2C8) - Azide and BSA Free [NBP2-72652] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Methylmalonyl Coenzyme A.")
![Immunocytochemistry/Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody (OTI2C8) - Azide and BSA Free [NBP2-72652] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Methylmalconyl Coenzyme A mutase.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-OTI2C8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72652-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody (OTI2C8) - Azide and BSA Free [NBP2-72652] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-OTI2C8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72652-img0003.jpg)
![Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against human RNLS (AA: 68-242) recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-Western-Blot-NBP2-52554-img0001.jpg "Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against human RNLS (AA: 68-242) recombinant protein. (Expected MW is 45.7 kDa)")
![Western Blot: Renalase Antibody (1C11E8) [NBP2-52554] - Analysis using RNLS mAb against HEK293 (1) and RNLS (AA: 68-242)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-Western-Blot-NBP2-52554-img0002.jpg)
![ELISA: Renalase Antibody (1C11E8) [NBP2-52554] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Renalase-Antibody-1C11E8-ELISA-NBP2-52554-img0003.jpg)
![Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-Western-Blot-NBP2-37562-img0003.jpg "Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: PGC1 alpha Antibody (1G8) [NBP2-37562] - Analysis using PGC1 alpha Antibody (1G8) against human PGC-1alpha (AA: 570-798) recombinant protein. Expected molecular weight is 70 KDa.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-Western-Blot-NBP2-37562-img0002.jpg)
![ELISA: PGC1 alpha Antibody (1G8) [NBP2-37562] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody-1G8-ELISA-NBP2-37562-img0001.jpg)
![Immunohistochemistry: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Adenylosuccinate Synthase antibody (NBP3-32005) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32005) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32005_rabbit-adenylosuccinate-synthase-mab-je64-42-298202413585560.jpg "Immunohistochemistry: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Adenylosuccinate Synthase antibody (NBP3-32005) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32005) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005] - Western blot analysis of Adenylosuccinate Synthase on different lysates with Rabbit anti-Adenylosuccinate Synthase antibody (NBP3-32005) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: U87MG cell lysate Lane 3: HL-60 cell lysate Lane 4: Mouse kidney tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32005) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32005_rabbit-adenylosuccinate-synthase-mab-je64-42-392024845354.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-97726-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-YWHAE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :30 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-97726-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-97726] - Immunofluorescence staining of 14-3-3 epsilon in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97726 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97726_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511545662.png)
![Western Blot: LYPLAL1 Antibody [NBP3-06252] - Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilutionLane A: COCLO205 Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251MG Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Western-Blot-NBP3-06252-img0001.jpg "Western Blot: LYPLAL1 Antibody [NBP3-06252] - Anti-LYPLAL1 rabbit polyclonal antibody at 1:500 dilutionLane A: COCLO205 Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251MG Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa")
![Immunohistochemistry-Paraffin: LYPLAL1 Antibody [NBP3-06252] - Immunochemical staining of human LYPLAL1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Immunohistochemistry-Paraffin-NBP3-06252-img0002.jpg)
![Immunoprecipitation: LYPLAL1 Antibody [NBP3-06252] - LYPLAL1 was immunoprecipitated using:Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-LYPLAL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-LYPLAL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :29 kDa](http://images.novusbio.com/fullsize/LYPLAL1-Antibody-Immunoprecipitation-NBP3-06252-img0003.jpg)
![Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-278202416331235.jpg "Western Blot: LAP3 Antibody (PSH01-34) [NBP3-32526] - Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: U-87 MG cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: LO2 cell lysate (20 ug/Lane) Lane 8: Mouse liver tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAP3 Antibody (PSH01-34) [NBP3-32526] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LAP3 antibody (NBP3-32526) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32526) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32526_rabbit-lap3-mab-psh01-34-298202414333666.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-298202414333655.jpg "Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 65 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31996) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-392024848509.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Western-Blot-NBP2-89374-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Immunoprecipitation-NBP2-89374-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89374 (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89374_rabbit-monoclonal-leukotriene-a4-hydrolase-lta4h-antibody-201-235202512205518.png)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-278202415141717.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Immunocytochemistry analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1 with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202410234298.jpg)
![Flow Cytometry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Flow cytometric analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32019, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202416453266.jpg)
![Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-278202416285222.jpg "Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling LRP2 with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32545, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492681.jpg)
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32545) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492624.jpg)