| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77459SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54399-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59779 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70531 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77459-img0008.jpg "Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - Analysis of heme oxygenase mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-NBP1-77459-img0007.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0005.jpg "Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP1-31241] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31241-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP1-31241] - OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-31241-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0003.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0004.jpg)
![Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP2-16769] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Guanine-deaminase-Antibody-Immunohistochemistry-Paraffin-NBP2-16769-img0002.jpg "Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP2-16769] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.")
![Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Western-Blot-NBP2-53271-img0002.jpg "Western Blot: S100B Antibody (SPM354) [NBP2-53271] - Western blot analysis of human brain lysate using S100B Mouse Monoclonal Antibody (SPM354).")
![Immunohistochemistry-Paraffin: S100B Antibody (SPM354) [NBP2-53271] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (SPM354).](http://images.novusbio.com/fullsize/S100B-Antibody-SPM354-Immunohistochemistry-Paraffin-NBP2-53271-img0001.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0011.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0008.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in HepG2.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0009.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in Raw 264.7.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0010.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0012.jpg)
![ELISA: NADK Antibody (5F4) [H00065220-M01] - Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-ELISA-H00065220-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - FAH antibody [HL1972] detects FAH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. FAH stained by FAH antibody [HL1972] (NBP3-25461) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249362768.jpg "Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - FAH antibody [HL1972] detects FAH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. FAH stained by FAH antibody [HL1972] (NBP3-25461) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1972] (NBP3-25461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249403839.jpg)
![Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1972] (NBP3-25461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249425438.jpg)
![Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - FAH antibody [HL1972] detects FAH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. FAH stained by FAH antibody [HL1972] (NBP3-25461) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249351330.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1972] (NBP3-25461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249371591.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarylacetoacetate hydrolase Antibody (HL1972) - Azide and BSA Free [NBP3-25461] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FAH antibody [HL1972] (NBP3-25461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25461_rabbit-fumarylacetoacetate-hydrolase-mab-hl1972-azide-bsa-free-2120249374956.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0002.jpg "Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (15 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody (BLR103H) [NBP3-14695] - Rabbit anti-GAPDH recombinant monoclonal antibody. Secondary HRP-conjugated goat anti-rabbit IgG. Substrate:DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Immunohistochemistry-NBP3-14695-img0003.jpg)
![Western Blot: GAPDH Antibody (BLR103H) [NBP3-14695] - Whole cell lysate (50 ug) from HeLa, Jurkat, RKO, U2OS, GaMG, LNCaP, MCF-7, K-562, HEK293T, and Hep-G2 cells prepared using NETN lysis buffer. Rabbit anti-GAPDH recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/GAPDH-Antibody-BLR103H-Western-Blot-NBP3-14695-img0001.jpg)
![ICC/IF-GAPDH Antibody (BLR103H)-NBP3-14695-: Rabbit anti-GAPDH recombinant monoclonalantibody [BLR103H]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14695_rabbit-gapdh-mab-immunocytochemistry-immunofluorescence-199202311112..jpg)
![Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518415223.jpg "<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518335915.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842516.jpg)
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251839419.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-1411202518392014.jpg)
![P2Y2 antibody [HL2704] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-immunocytochemistry-immunofluorescence-1411202518333113.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (NBP3-48721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48721_rabbit-p2y2-mab-hl2704-western-blot-141120251842412.jpg)
![Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human and mouse MafA by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or c-Maf using this antibody, rabbit anti-MafB recombinant monoclonal [BLR046F], and rabbit anti-c-Maf recombinant monoclonal [BLR045F]. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ). Lower panel: Rabbit anti-Myc.](http://images.novusbio.com/fullsize/MafA-Antibody-BLR067G-NBP2-78707-img0007.jpg "Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human and mouse MafA by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or c-Maf using this antibody, rabbit anti-MafB recombinant monoclonal [BLR046F], and rabbit anti-c-Maf recombinant monoclonal [BLR045F]. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ). Lower panel: Rabbit anti-Myc.")
![Immunocytochemistry/Immunofluorescence: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/MafA-Antibody-BLR067G-Immunocytochemistry-Immunofluorescence-NBP2-78707-img0009.jpg)
![Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/MafA-Antibody-BLR067G-Immunohistochemistry-Paraffin-NBP2-78707-img0008.jpg)
![Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of mouse MafA by WB of Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ).](http://images.novusbio.com/fullsize/MafA-Antibody-BLR067G-NBP2-78707-img0006.jpg)
![Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human MafA (red) in FFPE pancreatic carcinoma by IHC-IF using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/MafA-Antibody-BLR067G-NBP2-78707-img0004.jpg)
![Western Blot: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2E1.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-5B9-Western-Blot-NBP2-02155-img0001.jpg "Western Blot: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2E1.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) [NBP2-02155] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2E1.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-5B9-Immunocytochemistry-Immunofluorescence-NBP2-02155-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded Ovary tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-5B9-Immunohistochemistry-Paraffin-NBP2-02155-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Immunofluorescent staining of HepG2 cells using anti-Cytochrome P450 2E1mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-5B9-Immunocytochemistry-Immunofluorescence-NBP2-02155-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded endometrium tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2E1-Antibody-5B9-Immunohistochemistry-Paraffin-NBP2-02155-img0003.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)