| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16013-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98267-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-138SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26172-50ul | |||
![Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Western-Blot-NBP2-49213-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Analysis using Anti-FASN antibody NBP2-49213 (A) shows similar pattern to independent antibody NBP1-84733 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0003.jpg)
![Immunohistochemistry: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-NBP2-49213-img0001.jpg)
![Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP2-49213] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-Immunohistochemistry-Paraffin-NBP2-49213-img0002.jpg)
![Western Blot: Apolipoprotein CIII Antibody (3T7Y5) [NBP3-16093] - Western blot analysis of extracts of Human plasma cells, using Apolipoprotein CIII antibody (NBP3-16093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-3T7Y5-Western-Blot-NBP3-16093-img0005.jpg "Western Blot: Apolipoprotein CIII Antibody (3T7Y5) [NBP3-16093] - Western blot analysis of extracts of Human plasma cells, using Apolipoprotein CIII antibody (NBP3-16093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: Apolipoprotein CIII Antibody (3T7Y5) [NBP3-16093] - Immunofluorescence analysis of mouse liver using Apolipoprotein CIII Rabbit mAb (NBP3-16093) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-3T7Y5-Immunohistochemistry-NBP3-16093-img0004.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody (3T7Y5) [NBP3-16093] - Immunohistochemistry of paraffin-embedded human liver using Apolipoprotein CIII Rabbit mAb (NBP3-16093) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-3T7Y5-Immunohistochemistry-Paraffin-NBP3-16093-img0001.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421262933.jpg "Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: ABCG4 Antibody [NBP3-41376] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41376; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-61220242116286.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Human Hela cell lysate; Primary Ab: 1ug/ml NBP3-41376; Second Ab: 0.2ug/mLÂ HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421175964.jpg)
![Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Staining of Mouse Adrenal Gland lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Western-Blot-NBP1-00227-img0003.jpg "Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Staining of Mouse Adrenal Gland lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Immunohistochemistry-Paraffin-NBP1-00227-img0002.jpg)
![Immunohistochemistry-Paraffin: Adiponutrin/PNPLA3 Antibody [NBP1-00227] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Immunohistochemistry-Paraffin-NBP1-00227-img0001.jpg)
![Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-54611] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Western-Blot-NBP1-54611-img0003.jpg "Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-54611] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: Adiponutrin/PNPLA3 Antibody [NBP1-54611] - Human Heart Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Myocardial cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Immunohistochemistry-Paraffin-NBP1-54611-img0002.jpg)
![Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-54611] - Jurkat cell lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Western-Blot-NBP1-54611-img0001.jpg)
![Western Blot: Adiponutrin/PNPLA3 Antibody [NBP1-54611] - Reccomended Titration: 1 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Adiponutrin-PNPLA3-Antibody-Western-Blot-NBP1-54611-img0004.jpg)
![Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of various cell lines, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/OXPAT-Antibody-Western-Blot-NBP3-16013-img0002.jpg "Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of various cell lines, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: OXPAT Antibody [NBP3-16013] - Western blot analysis of extracts of HepG2 cells, using OXPAT antibody (NBP3-16013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/OXPAT-Antibody-Western-Blot-NBP3-16013-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: OXPAT Antibody - Azide and BSA Free [NBP3-16013] - Immunofluorescence analysis of HepG2 cells using OXPAT Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16013_rabbit-oxpat-pab-72202512443378.jpg)
![Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-288202412493476.jpg "Western Blot: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32977) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32977) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-2982024149263.jpg)
![Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody (PSH04-61) [NBP3-32977] - Immunocytochemistry analysis of HeLa cells labeling SREBP1 with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SREBP1 antibody (NBP3-32977) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32977_rabbit-srebp1-mab-psh04-61-29202410484870.jpg)
![Immunohistochemistry-Paraffin: PLA2G3 Antibody - BSA Free [NB100-59031] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/PLA2G3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59031-img0003.jpg "Immunohistochemistry-Paraffin: PLA2G3 Antibody - BSA Free [NB100-59031] - Brain, Glioblastoma")
![Immunohistochemistry-Paraffin: PLA2G3 Antibody - BSA Free [NB100-59031] - Analysis of anti-PLA2G3 antibody with human colon carcinoma at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PLA2G3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59031-img0001.jpg)
![Immunohistochemistry-Paraffin: PLA2G3 Antibody - BSA Free [NB100-59031] - Brain, Hypothalamus, neurons](http://images.novusbio.com/fullsize/PLA2G3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59031-img0002.jpg)
![Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Western-Blot-NBP2-98267-img0005.jpg "Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Lane A: Mouse liver tissue lysateLane B: Mouse skeletal muscle tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:54 kDa")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0004.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0002.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-98267] - Immunochemical staining of mouse Angiopoietin-like Protein 4/ANGPTL4 in mouse pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-4-ANGPTL4-Antibody-Immunohistochemistry-Paraffin-NBP2-98267-img0003.jpg)
![Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-262202415475620.jpg "Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: LDLR Antibody (2B10) [NBP3-26172] - Image of LDLR Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-27220248313719.jpg)
![Immunocytochemistry/Immunofluorescence: LDLR Antibody (2B10) [NBP3-26172] - Staining of Hela Cells with LDLR Antibody (2B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-282202412493641.jpg)
![Flow Cytometry: LDLR Antibody (2B10) [NBP3-26172] - Overlay histogram showing Hela cells stained with LDLR Antibody (2B10) (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26172_rabbit-ldlr-mab-2b10-282202414183657.jpg)