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![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-90359] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90359-img0007.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-90359] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-90359] - Staining of human hippocampus shows cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-90359-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining in human heart muscle and duodenum tissues using anti-FABP3 antibody. Corresponding FABP3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining in human heart muscle and duodenum tissues using anti-FABP3 antibody. Corresponding FABP3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FABP3/H-FABP Antibody [NBP2-33763] - Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33763-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0004.jpg)
![Immunohistochemistry-Paraffin: FABP3/H-FABP Antibody [NBP2-33763] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/FABP3-H-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-33763-img0005.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-20871-img0001.jpg "Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: PMP70 Antibody [NBP1-20871] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PMP70-Antibody-Flow-Cytometry-NBP1-20871-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NB300-176-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.")
![Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-2682024159393.jpg "Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Western blot analysis of Tryptophan hydroxylase 2 on different lysates with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/1,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 18 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-28820241249341.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33037) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-29820241492660.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421262933.jpg "Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: ABCG4 Antibody [NBP3-41376] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41376; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-61220242116286.jpg)
![Western Blot: ABCG4 Antibody [NBP3-41376] - Sample: Human Hela cell lysate; Primary Ab: 1ug/ml NBP3-41376; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41376_rabbit-abcg4-pab-612202421175964.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in mouse brain.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0005.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in mouse brain.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in human colon.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0003.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in human placenta.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0004.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [H00000759-D01P] - Analysis of CA1 expression in transfected 293T cell line by CA1 polyclonal antibody.Lane 1: CA1 transfected lysate(28.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-H00000759-D01P-img0006.jpg)
![Western Blot: MARCKS Antibody [NBP3-05540] - Western blot analysis of different cell lines lysates using MARCKS, at a dilution of 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH at a dilution of 1:5,000 in red, which reveals a single band at 37kDa in all preparations.](http://images.novusbio.com/fullsize/MARCKS-Antibody-Western-Blot-NBP3-05540-img0002.jpg "Western Blot: MARCKS Antibody [NBP3-05540] - Western blot analysis of different cell lines lysates using MARCKS, at a dilution of 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH at a dilution of 1:5,000 in red, which reveals a single band at 37kDa in all preparations.")
![Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05540] - Immunofluorescent analysis of HeLa cells stained with MARCKS Antibody at a dilution of 1:5,000 in red, and costained with mouse mAb to beta-tubulin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the beta-tubulin antibody stains cytoplasmic microtubules.](http://images.novusbio.com/fullsize/MARCKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05540-img0001.jpg)
![Western Blot: MARCKS Antibody [NBP3-05539] - Western blot analysis of different cell lysates using MARCKS Antibody in green: [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6 and [7] COS1 cells. The band at about 80kDa corresponds to the MARCKS protein detected only in the human and monkey cells. The antibody does not recognize the rodent form of the MARCKS protein.](http://images.novusbio.com/fullsize/MARCKS-Antibody-Western-Blot-NBP3-05539-img0002.jpg "Western Blot: MARCKS Antibody [NBP3-05539] - Western blot analysis of different cell lysates using MARCKS Antibody in green: [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6 and [7] COS1 cells. The band at about 80kDa corresponds to the MARCKS protein detected only in the human and monkey cells. The antibody does not recognize the rodent form of the MARCKS protein.")
![Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05539] - Immunofluorescent analysis of HeLa cell culture stained with MARCKS Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. MARCKS antibody binds to MARCKS protein expressed in the membrane structures and in cytoplasm, while the vimentin antibody stains the intermediate filaments.](http://images.novusbio.com/fullsize/MARCKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05539-img0001.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740] - (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-NBP1-36740-img0002.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740] - (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Click](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-36740-img0001.jpg)
![Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml).](http://images.novusbio.com/fullsize/PDHX-Antibody-Western-Blot-NB100-1496-img0003.jpg "Western Blot: PDHX Antibody [NB100-1496] - Western blot analysis of Human Pancreas lysate (35ug protein in RIPA buffer) using NB100-1496 (1ug/ml).")
![Immunohistochemistry: PDHX Antibody [NB100-1496] - staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3ug/ml), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1ug/ml) , HRP-staining.](http://images.novusbio.com/fullsize/PDHX-Antibody-Immunohistochemistry-NB100-1496-img0001.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-p-Ser293-Antibody-9S3I4-Immunoprecipitation-NBP3-16916-img0002.jpg "Immunoprecipitation: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916). Western blot was performed from the immunoprecipitate using Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-16916) at a dilition of 1:1000.")
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at1:23000 dilution.NIH/3T3 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483541..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (9S3I4) [NBP3-16916] -analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda -PP mixed solution (1ul) at 30 c for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25uμg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-western-blot-75202483713..jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (6E3J0) [NBP3-16916] - Western blot analysis of lysates from HeLa cells, using Pyruvate Dehydrogenase E1-alpha subunit Rabbit mAb at 1:23000 dilution.293T cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16916_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser-293-mab-6e3j0-52202511383736.jpg)