| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03703-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38063-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26568-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22133-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39029-25ul | |||
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48666_rabbit-ferritin-heavy-chain-mab-hl1905-western-blot-1411202518351214.jpg "Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48666_rabbit-ferritin-heavy-chain-mab-hl1905-western-blot-1411202518363623.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48666_rabbit-ferritin-heavy-chain-mab-hl1905-western-blot-1411202518321623.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48666_rabbit-ferritin-heavy-chain-mab-hl1905-western-blot-141120251840439.jpg)
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-Western-Blot-NBP1-57716-img0006.jpg "Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.")
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-Western-Blot-NBP1-57716-img0003.jpg)
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-Western-Blot-NBP1-57716-img0004.jpg)
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-Western-Blot-NBP1-57716-img0005.jpg)
![Western Blot: Apolipoprotein A-II/ApoA2 Antibody [NBP1-57716] - Human HepG2, Antibody Dilution: 1.0 ug/ml APOA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-Western-Blot-NBP1-57716-img0007.jpg)
![Western Blot: GADD34 Antibody [NBP3-03703] - Analysis of extracts of various cell lines, using GADD34 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GADD34-Antibody-Western-Blot-NBP3-03703-img0001.jpg "Western Blot: GADD34 Antibody [NBP3-03703] - Analysis of extracts of various cell lines, using GADD34 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] - Immunofluorescence analysis of H9C2 cells using GADD34 Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03703_rabbit-polyclonal-gadd34-antibody-5220251931361.jpg)
![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] - Immunofluorescence analysis of L929 cells using GADD34 Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03703_rabbit-polyclonal-gadd34-antibody-52202519294595.jpg)
![Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Immunofluorescence analysis of L929 cells using Methylmalonyl Coenzyme A mutase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38063_rabbit-methylmalonyl-coenzyme-a-mutase-pab-91220241519564.jpg "Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Immunofluorescence analysis of L929 cells using Methylmalonyl Coenzyme A mutase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Immunofluorescence analysis of U-2 OS cells using Methylmalonyl Coenzyme A mutase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38063_rabbit-methylmalonyl-coenzyme-a-mutase-pab-912202414123817.jpg)
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP3-38063] - Western blot analysis of various lysates using Methylmalonyl Coenzyme A mutase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-38063_rabbit-methylmalonyl-coenzyme-a-mutase-pab-912202414213713.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody [H00004594-B01P] - Analysis of MUT expression in human liver.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Western-Blot-H00004594-B01P-img0004.jpg "Western Blot: Methylmalonyl Coenzyme A mutase Antibody [H00004594-B01P] - Analysis of MUT expression in human liver.")
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody [H00004594-B01P] - Analysis of MUT expression in A-431.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Western-Blot-H00004594-B01P-img0003.jpg)
![Western Blot: Methylmalonyl Coenzyme A mutase Antibody [H00004594-B01P] - Analysis of MUT expression in transfected 293T cell line by MUT polyclonal antibody. Lane 1: MUT transfected lysate(82.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Methylmalonyl-Coenzyme-A-mutase-Antibody-Western-Blot-H00004594-B01P-img0005.jpg)
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/MID1-Antibody-Western-Blot-NBP1-26612-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.")
![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-26220241447157.jpg "Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-27220248573313.jpg)
![Western Blot: Ferritin Antibody (12H1) [NBP3-26568] - Positive Western Blot detected in: U251 whole cell lysate, JK whole cell lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202411485815.jpg)
![Flow Cytometry: Ferritin Antibody (12H1) [NBP3-26568] - Overlay Peak curve showing 293 cells stained with Ferritin Antibody (12H1) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202414312738.jpg)
![Western Blot: ABCE1 Antibody (SR2116) [NBP3-22133] - Western blot analysis using NBP3-22133 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22133_rabbit-abce1-mab-sr2116-57202316494255.jpg "Western Blot: ABCE1 Antibody (SR2116) [NBP3-22133] - Western blot analysis using NBP3-22133 in HeLa cell lysate.")
![Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin Heavy Chain in K562, CHO-K1, Hela cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Western-Blot-NBP3-15028-img0002.jpg "Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin Heavy Chain in K562, CHO-K1, Hela cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.")
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Immunofluorescence of Ferritin Heavy Chain (green) in Hela cells using Ferritin Rabbit mAb at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Immunocytochemistry-Immunofluorescence-NBP3-15028-img0003.jpg)
![Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin in Raw264. 7 cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Western-Blot-NBP3-15028-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39029-img0002.jpg "Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0004.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0003.jpg)