| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87293-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76735-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95253-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0002.jpg "Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunocytochemistry-Immunofluorescence-NBP3-13632-img0003.jpg)
![Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunohistochemistry-Paraffin-NBP3-13632-img0004.jpg)
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]Detection of human IDH2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-78202410372610.jpg "Immunocytochemistry/ Immunofluorescence;IDH2 Antibody[NBP3-29974]Detection of human IDH2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.")
![Immunohistochemistry;IDH2 Antibody[NBP3-29975]Detection of mouse IDH2 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-7820241039269.jpg)
![Immunohistochemistry;IDH2 Antibody[NBP3-29996]Detection of human IDH2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma and lung cancer . Antibody: Affinity purified rabbit anti-IDH2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-782024936138.jpg)
![Immunoprecipitation:IDH2 AntibodyNBP3-29996] -Detection of human IDH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody NBP3-29996 (lot NBP3-29996-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated IDH2, NBP3-29996 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29996_rabbit-idh2-pab-3012202419525811.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249432858.jpg "Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. PFKM stained by PFKM antibody [HL2010] (NBP3-25599) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-21202494149.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-212024941459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (HL2010) - Azide and BSA Free [NBP3-25599] - Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-2120249422638.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Muscle Phosphofructokinase/PFKM/PFK-1 antibody [HL2010] (NBP3-25599) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25599_rabbit-muscle-phosphofructokinase-pfkm-pfk-1-mab-hl2010-azide-bsa-free-western-blot-1411202518312123.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25353_rabbit-perilipin-2-adfp-mab-hl2147-azide-and-bsa-free-2120249342760.jpg "Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25353_rabbit-perilipin-2-adfp-mab-hl2147-azide-and-bsa-free-2120249393511.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - ADFP antibody [HL2147] detects ADFP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ADFP stained by ADFP antibody [HL2147] (NBP3-25353) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25353_rabbit-perilipin-2-adfp-mab-hl2147-azide-and-bsa-free-2120249425441.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (HL2147) - Azide and BSA Free [NBP3-25353] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (NBP3-25353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25353_rabbit-perilipin-2-adfp-mab-hl2147-azide-and-bsa-free-2120249351336.jpg)
![ADFP antibody [HL2147] detects ADFP protein at lipid droplet by immunofluorescent analysis.Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ADFP stained by ADFP antibody [HL2147] (NBP3-25353) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25353_rabbit-perilipin-2-adfp-mab-hl2147-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2611202518202315.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0012.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87293-img0013.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87293-img0010.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP1-87293] - Analysis in human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP1-87293-img0011.jpg)
![Western Blot: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue lysate with HSL antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-Antibody-Western-Blot-NBP1-76735-img0003.jpg "Western Blot: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue lysate with HSL antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunohistochemistry-Paraffin: Hormone-sensitive Lipase/HSL Antibody [NBP1-76735] - Human lymph node tissue with HSL antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Hormone-sensitive-Lipase-HSL-Antibody-Immunohistochemistry-Paraffin-NBP1-76735-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Hormone-sensitive Lipase/HSL Antibody - BSA Free [NBP1-76735] - Immunofluorescence of Hormone-sensitive Lipase/HSL in Human Lymph Node cells with Hormone-sensitive Lipase/HSL antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76735_rabbit-polyclonal-hormone-sensitive-lipase-hsl-antibody-271120242011550.jpg)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-western-blot-241202412935..jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - analysis of various lysates, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.5s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] -Analysis of paraffin-embedded rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121118..jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95253] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-95253_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-immunohistochemistry-paraffin-2412024121229..jpg)
![Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Western-Blot-NBP3-15366-img0004.jpg "Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of NIH-3T3 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0003.jpg)
![Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunohistochemistry-Paraffin-NBP3-15366-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of C6 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0002.jpg)
![Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-644-img0002.jpg "Western Blot: PINK1 Antibody [NB100-644] - Analysis of C-terminally V5His-tagged human PINK1 or N-teminally myc-tagged human PINK1 expressed in HEK293T cells using NB100-644 at 1:1000 dilution. Observed molecular weight ~64 kDa.")
![Simple Western: PINK1 Antibody [NB100-644] - Simple Western lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~56 kDa. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NB100-644-img0003.jpg)
![Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-27820241633128.jpg "Western Blot: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32596) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-298202413585565.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32596, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29820241430416.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-18) [NBP3-32596] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32596) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32596_rabbit-mitofilin-mab-psh01-18-29202410334040.jpg)
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414304127.jpg "Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunofluorescence analysis of paraffin-embedded human thyroid carcinoma tissue labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32597, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-298202414333672.jpg)
![Immunocytochemistry/ Immunofluorescence: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Immunocytochemistry analysis of HeLa cells labeling Mitofilin with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-29202410334014.jpg)
![Western Blot: Mitofilin Antibody (PSH01-19) [NBP3-32597] - Western blot analysis of Mitofilin on different lysates with Rabbit anti-Mitofilin antibody (NBP3-32597) at 1/100,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HEK-293 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Saos-2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32597_rabbit-mitofilin-mab-psh01-19-3920248485018.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)