| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92746-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92339-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89830-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89736-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84782-25ul | |||
![Western Blot: TRIM5 Antibody [NB100-1226] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TRIM5-Antibody-Western-Blot-NB100-1226-img0001.jpg "Western Blot: TRIM5 Antibody [NB100-1226] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.](http://images.novusbio.com/fullsize/TRIM5-Antibody-Immunohistochemistry-Paraffin-NB100-1226-img0003.jpg)
![Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Staining of paraffin embedded Human Colon. Antibody at 3 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TRIM5-Antibody-Immunohistochemistry-Paraffin-NB100-1226-img0002.jpg)
![Western Blot: Cytochrome P450 2B6 Antibody [NBP2-92746] - Analysis of extracts of various cell lines, using Cytochrome P450 2B6 .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-Western-Blot-NBP2-92746-img0004.jpg "Western Blot: Cytochrome P450 2B6 Antibody [NBP2-92746] - Analysis of extracts of various cell lines, using Cytochrome P450 2B6 .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody [NBP2-92746] - Paraffin-embedded mouse lung using Cytochrome P450 2B6 .](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-Immunohistochemistry-Paraffin-NBP2-92746-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody [NBP2-92746] - Paraffin-embedded human breast cancer using Cytochrome P450 2B6 .](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-Immunohistochemistry-Paraffin-NBP2-92746-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody [NBP2-92746] - Paraffin-embedded human colon using Cytochrome P450 2B6 .](http://images.novusbio.com/fullsize/Cytochrome-P450-2B6-Antibody-Immunohistochemistry-Paraffin-NBP2-92746-img0002.jpg)
![Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.](http://images.novusbio.com/fullsize/ABCA2-Antibody-Western-Blot-NBP2-92339-img0003.jpg "Western Blot: ABCA2 Antibody [NBP2-92339] - Analysis of extracts of various cell lines, using ABCA2 .Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of paraffin-embedded mouse brain using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-4220251429023.jpg%3Ft=1709177288)
![Immunocytochemistry/ Immunofluorescence: ABCA2 Antibody - Azide and BSA Free [NBP2-92339] - Immunofluorescence analysis of U-251 MG cells using ABCA2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92339_rabbit-polyclonal-abca2-antibody-42202514264481.jpg%3Ft=1709177288)
![Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Caveolin-1-Antibody-9I9J8-Western-Blot-NBP3-15615-img0017.jpg "Western Blot: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Western blot analysis of extracts of HeLa cells, using Caveolin-1antibody (NBP3-15615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] - Human colon using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410253..jpg)
![Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-immunohistochemistry-paraffin-184202410355..jpg)
![Western Blot: Caveolin-1 Antibody (10Y5L2) [Caveolin-1] - Western blot analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15615_rabbit-caveolin-1-mab-10y5l2-5220251731106.jpg)
![Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-064-Western-Blot-NBP2-89830-img0001.jpg "Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa")
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511545677.png)
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511444489.png)
![Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDa](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-23520251154565.png)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Immunohistochemistry-Paraffin-NBP2-67164-img0002.jpg)
![Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Flow-Cytometry-NBP2-67164-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0004.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Western-Blot-NBP2-89736-img0002.jpg "Western Blot: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDa Observed band size:53 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Analysis of Human AGT expression on HepG2 cells. The cells were treated ,stained with purified anti-Human AGT, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Flow-Cytometry-NBP2-89736-img0004.jpg)
![Immunoprecipitation: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SerpinA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Serpin-A8-Angiotensinogen-Antibody-1-Immunoprecipitation-NBP2-89736-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody (1) [NBP2-89736] - Staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89736 antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89736_rabbit-monoclonal-serpin-a8-angiotensinogen-antibody-001-23520251129370.png)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-84782-img0011.jpg "Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Staining of human liver shows no positivity in hepatocytes as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Analysis in human prostate and liver tissues. Corresponding MRP4/ABCC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-84782-img0008.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Staining of human fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-84782-img0009.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-84782-img0010.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NBP1-84782] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-84782-img0012.jpg)