| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71422 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77217-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74462 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54319-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38460-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35805-20ul | |||
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0007.jpg "Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI (whey protein isolate), WPH (whey peptide hydrolysate), BPI (BSA protein isolate) or BPH (BSA peptide hydrolysate). Immunoblotting and densitometric analysis of (A,D) adiponectin (n = 6). mRNA levels of (F) adiponectin (n = 8) were determined. * p < 0.05, **** p < 0.0001 vs. protein isolate controls. PS, protein stain. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32041341) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of Adiponectin was performed using 10% confluent 3T3-L1 cells differentiated with Adipogenesis supplement for 5 days.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunocytochemistry-Immunofluorescence-NBP2-22450-img0011.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed on biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-Paraffin-NBP2-22450-img0010.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed using 10 ul of conditioned media of Hep G2 cell line (lane 1).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0009.jpg)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-NBP2-22450-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Adipocytes isolated from the pelvic area of the male mouse. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-22450_mouse-monoclonal-adiponectin-acrp30-antibody-19f1-immunocytochemistry-immunofluorescence-1111202494957..jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Western-Blot-NBP2-01418-img0019.jpg "Western Blot: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponectin.")
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) [NBP2-01418] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunocytochemistry-Immunofluorescence-NBP2-01418-img0011.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0018.jpg)
![Flow Cytometry: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adiponectin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Flow-Cytometry-NBP2-01418-img0010.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0012.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0014.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0015.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0016.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0017.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody [NBP1-77217] - analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-Western-Blot-NBP1-77217-img0004.jpg "Western Blot: Adiponectin/Acrp30 Antibody [NBP1-77217] - analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody - BSA Free [NBP1-77217] - Immunofluorescence of Adiponectin/Acrp30 in Rat Brain cells with Adiponectin/Acrp30 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77217_rabbit-polyclonal-adiponectin-acrp30-antibody-2711202419562116.jpg)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody - BSA Free [NBP1-77217] - Immunohistochemistry of Adiponectin in mouse brain tissue with Adiponectin antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-77217_rabbit-polyclonal-adiponectin-acrp30-antibody-271120242045631.gif)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody - BSA Free [NBP1-77217] - Immunohistochemistry of adiponectin in rat brain tissue with adiponectin antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77217_rabbit-polyclonal-adiponectin-acrp30-antibody-2711202420175199.jpg)
![Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody - BSA Free [NBP1-77217] - Immunofluorescence of Adiponectin/Acrp30 in mouse brain tissue with Adiponectin/Acrp30 antibody at 20 ug/mL.Red: Adiponectin/Acrp30 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77217_rabbit-polyclonal-adiponectin-acrp30-antibody-271120242022916.gif)
![Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Western-Blot-NBP2-00597-img0004.jpg "Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.")
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Immunocytochemistry-Immunofluorescence-NBP2-00597-img0006.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0003.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0001.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0002.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody [NBP2-38657] -Analysis in human breast tissue.](http://images.novusbio.com/fullsize/antibody/nbp2-38657_rabbit-polyclonal-adiponectin-acrp30-antibody-western-blot-214202594454..png "Western Blot: Adiponectin/Acrp30 Antibody [NBP2-38657] -Analysis in human breast tissue.")
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NBP2-38657] -Staining of human skeletal muscle shows strong membranous positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-38657_rabbit-polyclonal-adiponectin-acrp30-antibody-immunohistochemistry-paraffin-21420259461..png)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NBP2-38657] - Staining of human endometrium shows strong membranous positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/antibody/nbp2-38657_rabbit-polyclonal-adiponectin-acrp30-antibody-immunohistochemistry-paraffin-214202594651..png)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NBP2-38657] - Staining of human skin shows strong membranous positivity in basal squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp2-38657_rabbit-polyclonal-adiponectin-acrp30-antibody-immunohistochemistry-paraffin-214202594747..png)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NBP2-38657] -Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/antibody/nbp2-38657_rabbit-polyclonal-adiponectin-acrp30-antibody-immunohistochemistry-paraffin-214202594848..png)
![Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Western-Blot-NB100-57094-img0002.jpg "Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NB100-57094-img0003.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Western blot Analysis of human Kidney lysate using Adiponectin/Acrp30 Antibody (ADPN/1370).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Western-Blot-NBP2-47652-img0004.jpg "Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Western blot Analysis of human Kidney lysate using Adiponectin/Acrp30 Antibody (ADPN/1370).")
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Formalin-fixed, paraffin-embedded human Kidney stained with Adiponectin Monoclonal Antibody (ADPN/1370).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Immunohistochemistry-Paraffin-NBP2-47652-img0001.jpg)
![Protein Array: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Adiponectin/Acrp30 Antibody (ADPN/1370). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Protein-Array-NBP2-47652-img0003.jpg)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32003) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-2982024149262.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32003) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin/Acrp30 with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-29202410484876.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Western blot analysis of Adiponectin/Acrp30 on mouse colon tissue lysates with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32003) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-392024848505.jpg)
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38460-img0005.jpg "Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38460] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NBP2-38460-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)