| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35822-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37454-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37271-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37350-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41535SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44299SS | |||
| Azide and BSA Free | NBP2-80754 | |||
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152875.jpg "Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of C6 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415171113.jpg)
![Western Blot: PTRF Antibody [NBP3-35822] - Western blot analysis of various lysates using PTRF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415175297.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of U-2 OS cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414163015.jpg)
![Immunocytochemistry/ Immunofluorescence: PTRF Antibody [NBP3-35822] - Immunofluorescence analysis of L929 cells using PTRF Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202415152835.jpg)
![Immunohistochemistry: PTRF Antibody [NBP3-35822] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PTRF Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35822_rabbit-ptrf-pab-912202414123822.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0003.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Confocal imaging of HeLa cells using RXR alpha/NR2B1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-5220251731103.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR alpha/NR2B1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517344269.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 in the representative genomic region surrounding ECH1 gene.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517315534.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR alpha/NR2B1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517352787.jpg)
![Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Western-Blot-NBP2-37454-img0002.jpg "Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)")
![Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Immunocytochemistry-Immunofluorescence-NBP2-37454-img0003.jpg)
![Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of breast cancer tissues using PTPN11 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Immunohistochemistry-NBP2-37454-img0005.jpg)
![Flow Cytometry: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - An intracellular stain was performed on HeLa cells with SHP-2/PTPN11 Antibody (6D9) NBP2-37454F and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Flow-Cytometry-NBP2-37454-img0006.jpg)
![Flow Cytometry: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HepG2 cells using PTPN11 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-Flow-Cytometry-NBP2-37454-img0004.jpg)
![ELISA: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-6D9-ELISA-NBP2-37454-img0001.jpg)
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg)
![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Western-Blot-NBP2-37350-img0003.jpg "Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against HEK293 (1) and ABCC4 (AA: 631-692)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Analysis of cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Immunohistochemistry-NBP2-37350-img0006.jpg)
![Flow Cytometry: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - An intracellular stain was performed on A549 cells with MRP4/ABCC4 Antibody (2D2A9) NBP2-37350AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Flow-Cytometry-NBP2-37350-img0007.jpg)
![Western Blot: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Western blot analysis using ABCC4 mAb against human ABCC4 recombinant protein. (Expected MW is 32.4 kDa)](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Western-Blot-NBP2-37350-img0002.jpg)
![Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Analysis of endometrial cancer tissues using ABCC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Immunohistochemistry-NBP2-37350-img0005.jpg)
![Flow Cytometry: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Flow cytometric analysis of A549 cells using ABCC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-Flow-Cytometry-NBP2-37350-img0004.jpg)
![ELISA: MRP4/ABCC4 Antibody (2D2A9) [NBP2-37350] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MRP4-ABCC4-Antibody-2D2A9-ELISA-NBP2-37350-img0001.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0006.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0001.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human adrenal, pheochromocytoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0002.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0003.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas, islet of Langerhans, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0004.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0005.jpg)
![Immunohistochemistry: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of adrenal](http://images.novusbio.com/fullsize/GLP-1R Antibody-Immunohistochemistry-NBP3-14335-img0007.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Western blot analysis of CD39/ENTPD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Western-Blot-NBP2-67230-img0006.jpg "Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Western blot analysis of CD39/ENTPD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: mouse spleen tissue lysate")
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD39/ENTPDI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Immunohistochemistry-Paraffin-NBP2-67230-img0007.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human placenta tissue using anti-CD39 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Immunohistochemistry-Paraffin-NBP2-67230-img0001.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human spleen tissue using anti-CD39 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Immunohistochemistry-Paraffin-NBP2-67230-img0002.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human tonsil tissue using anti-CD39 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Immunohistochemistry-Paraffin-NBP2-67230-img0003.jpg)
![Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded mouse uterus tissue using anti-CD39 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD39-ENTPD1-Antibody-JA90-36-Immunohistochemistry-Paraffin-NBP2-67230-img0004.jpg)
![Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.](http://images.novusbio.com/fullsize/TREK-1-Antibody-Western-Blot-NB110-41535-img0003.jpg "Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.")
![Western Blot: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of partial recombinant GLUT4 protein using GLUT4 antibody [clone 2E11NB].](http://images.novusbio.com/fullsize/Glut4-Antibody-2E11NB-Western-Blot-NBP2-44299-img0002.jpg "Western Blot: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of partial recombinant GLUT4 protein using GLUT4 antibody [clone 2E11NB].")
![Immunohistochemistry-Paraffin: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of a FFPE tissue section of normal human skeletal muscle using 1:200 dilution of Glut4 antibody (clone 2E11NB). The antibody generated a diffused staining in the blood vessels as well as the cytoplasm/sarcoplasma of skeletal muscles while the nuclei did not show any positivity for Glut4.](http://images.novusbio.com/fullsize/Glut4-Antibody-2E11NB-Immunohistochemistry-Paraffin-NBP2-44299-img0003.jpg)
![Western Blot: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of human heart tissue lysate using 1:500 dilution of GLUT4 antibody [clone 2E11NB]. The antibody generated a single specific band of Glut4 at ~55 kDa molecular weight position.](http://images.novusbio.com/fullsize/Glut4-Antibody-2E11NB-Western-Blot-NBP2-44299-img0001.jpg)