| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85691-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48854-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93792-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89887-50ul | |||
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0026.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0021.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0025.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0027.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Simple Western lane view shows a specific band for CES1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0006.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Electropherogram image(s) of corresponding Simple Western lane view. CES1 antibody was used at 1:20 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0007.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex, liver, prostate and upper gastrointestinal using Anti-CES1 antibody NBP2-48854 (A) shows similar protein distribution across tissues to independent antibody NBP1-85691 (B).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis of human cell line Hep G2 shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48854-img0001.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0017.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0013.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0015.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0016.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0018.jpg)
![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0013.jpg "Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Immunohistochemistry-Paraffin-H00005004-M01-img0008.jpg)
![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0010.jpg)
![Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - ORM1 monoclonal antibody (M01), clone 2F9-1F10 Analysis of ORM1 expression in MCF-7.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Western-Blot-H00005004-M01-img0012.jpg)
![Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-Immunoprecipitation-H00005004-M01-img0009.jpg)
![ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Detection limit for recombinant GST tagged ORM1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-1-Acid-Glycoprotein-Antibody-2F9-1F10-ELISA-H00005004-M01-img0014.jpg)
![Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Nuclear Factor Erythroid 2 Related Factor 1 Antibody-Western Blot-NBP2-93792-img0009.jpg "Western Blot: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Western blot analysis of extracts of various cell lines, using Nuclear Factor Erythroid 2 Related Factor 1 antibody (NBP2-93792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Analysis of HeLa cells using Nuclear Factor Erythroid 2 Related Factor 1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93792-img0001.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded rat heart using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0008.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded human placenta using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0005.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid 2 Related Factor 1 Antibody [NBP2-93792] - Paraffin-embedded mouse brain using Nuclear Factor Erythroid 2 Related Factor 1 .](http://images.novusbio.com/fullsize/Nuclear-Factor-Erythroid-2-Related-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93792-img0007.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Western blot analysis of Carboxylesterase 1/CES1 on different lysates with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (10 ug/Lane) Lane 3: Human lung tissue lysate (10 ug/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32541) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-278202416331252.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Western blot analysis of Carboxylesterase 1/CES1 on different lysates with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (10 ug/Lane) Lane 3: Human lung tissue lysate (10 ug/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32541) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-298202414304183.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Immunocytochemistry analysis of U-937 cells labeling Carboxylesterase 1/CES1 with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Carboxylesterase 1/CES1 antibody (NBP3-32541) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-2920241192346.jpg)
![Flow Cytometry: Carboxylesterase 1/CES1 Antibody (JE33-04) [NBP3-32541] - Flow cytometric analysis of U-937 cells labeling Carboxylesterase 1/CES1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32541, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32541_rabbit-carboxylesterase-1-ces1-mab-je33-04-39202488460.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of various cell lines, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0002.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of various cell lines, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Immunofluorescence analysis of HeLa cells using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Immunocytochemistry-Immunofluorescence-NBP3-15388-img0001.jpg)
![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of HepG2 cells, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [Carboxylesterase 1/CES1] - Confocal imaging of paraffin-embedded Mouse liver tissue using Carboxylesterase 1/CES1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15388_rabbit-carboxylesterase-1-ces1-mab-7c9r6-52202517202984.jpg)
![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [Carboxylesterase 1/CES1] - Confocal imaging of paraffin-embedded Rat liver tissue using Carboxylesterase 1/CES1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15388_rabbit-carboxylesterase-1-ces1-mab-7c9r6-52202517284992.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0003.jpg "Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: COL4A3BP Antibody [NBP1-31118] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31118-img0002.jpg)
![Immunohistochemistry-Paraffin: COL4A3BP Antibody [NBP1-31118] - BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-31118-img0004.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - A: Hep G2 B: MOLT4 C: Raji 12% SDS PAGE; antibody diluted at 1:500](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0001.jpg)
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-61220242151820.jpg "Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Prostate cancer Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202421351278.jpg)
![Western Blot: Orosomucoid 2 Antibody (D7) [NBP3-41442] - Sample: Lane1: Human Serum; Lane2: Rat Liver lysate; Lane3: Rat Pancreas lysate; Lane4: Rat Bladder lysate Primary Ab: 3ug/ml NBP3-41442; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202420484723.jpg)
![Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CES1-Antibody-Western-Blot-NBP1-00167-img0005.jpg "Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CES1 Antibody [NBP1-00167] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-00167-img0004.jpg)
![Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/Serum-Amyloid-A4-Antibody-001-Western-Blot-NBP2-89887-img0003.jpg "Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Analysis of Human SAA4 expression on HepG2 cells. The cells were treated, stained with purified anti-Human SAA4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serum-Amyloid-A4-Antibody-001-Flow-Cytometry-NBP2-89887-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Staining of Human SAA4 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89887 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89887_rabbit-monoclonal-serum-amyloid-a4-antibody-001-23520251664777.png)
![Western Blot: Orosomucoid 2 Antibody [NBP3-32680] - Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 24 kDa Observed band size: 54 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32680) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-278202415242661.jpg "Western Blot: Orosomucoid 2 Antibody [NBP3-32680] - Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 24 kDa Observed band size: 54 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32680) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Orosomucoid 2 Antibody [NBP3-32680] - Immunocytochemistry analysis of HepG2 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-292024111487.jpg)
![Flow Cytometry: Orosomucoid 2 Antibody [NBP3-32680] - Flow cytometric analysis of HepG2 cells labeling Orosomucoid 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32680, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-29202416372170.jpg)
![Western Blot: COL4A3BP Antibody [NBP2-15943] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP2-15943-img0001.jpg "Western Blot: COL4A3BP Antibody [NBP2-15943] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: COL4A3BP Antibody [NBP2-15943] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15943-img0002.jpg)