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![Western Blot: ATF2 [p Ser490, p Ser498] Antibody [NB300-257]](http://images.novusbio.com/fullsize/ATF2-[p-Ser490--p-Ser498]-Antibody-Western-Blot-NB300-257-img0001.jpg "Western Blot: ATF2 [p Ser490, p Ser498] Antibody [NB300-257]")
![Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458] - Formalin fixed paraffin embedded breast carcinoma stained with Transglutaminase 2/TGM2 antibody (NBP2-26458).](http://images.novusbio.com/fullsize/Transglutaminase-2-TGM2-Antibody-CUB7402-Immunohistochemistry-Paraffin-NBP2-26458-img0003.jpg "Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458] - Formalin fixed paraffin embedded breast carcinoma stained with Transglutaminase 2/TGM2 antibody (NBP2-26458).")
![Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-MEM-188-Flow-Cytometry-NBP3-09126-img0001.jpg "Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.")
![Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-288202412442911.jpg "Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Immunocytochemistry analysis of L-929 cells treated with or without 20μM Z-VAD for 2.75 hours, add 100nM SM-164 and 20ng/ml TNF-alpha for 2.25 hours, then treated with lambda pp for 1 hour labeling RIPK3/RIP3 with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-29202410234210.jpg)
![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD15-Lewis-X-Antibody-MEM-158-Flow-Cytometry-NB500-376-img0003.jpg "Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.")
![Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.](http://images.novusbio.com/fullsize/CD15-Lewis-X-Antibody-MEM-158-Flow-Cytometry-NB500-376-img0001.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: IL-13 Antibody [NBP1-42776] - Analysis of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.](http://images.novusbio.com/fullsize/IL-13-Antibody-Western-Blot-NBP1-42776-img0004.jpg "Western Blot: IL-13 Antibody [NBP1-42776] - Analysis of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.")
![Immunohistochemistry-Paraffin: PRG2 Antibody [NBP2-19928] - Human hepatoma, using PRG2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PRG2-Antibody-Immunohistochemistry-Paraffin-NBP2-19928-img0002.jpg "Immunohistochemistry-Paraffin: PRG2 Antibody [NBP2-19928] - Human hepatoma, using PRG2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: ADAM33 Antibody [NBP1-30898] - Sample (30 ug of whole cell lysate)A: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ADAM33-Antibody-Western-Blot-NBP1-30898-img0002.jpg "Western Blot: ADAM33 Antibody [NBP1-30898] - Sample (30 ug of whole cell lysate)A: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: ADAM33 Antibody [NBP1-30898] - Paraffin-embedded placenta. ADAM33 antibody [C2C3], C-term dilution: 1:100.](http://images.novusbio.com/fullsize/ADAM33-Antibody-Immunohistochemistry-Paraffin-NBP1-30898-img0003.jpg)
![Western Blot: GIMAP5 Antibody [NBP1-31165] - Sample (30 ug of whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Western-Blot-NBP1-31165-img0002.jpg "Western Blot: GIMAP5 Antibody [NBP1-31165] - Sample (30 ug of whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: GIMAP5 Antibody [NBP1-31165] - Paraffin-embedded breast cancer. GIMAP5 antibody dilution: 1:250.](http://images.novusbio.com/fullsize/GIMAP5-Antibody-Immunohistochemistry-Paraffin-NBP1-31165-img0003.jpg)