| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61687-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52422-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37328-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28142 | |||
| FITC | NBP1-28141 | |||
| PE | NBP1-28143 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61863-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35252-1mg | |||
| Carrier-Free | NBP2-35252-100ug | |||
| Carrier-Free | NBP2-35252-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61726-0.025ml | |||
![Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Detection of CD20 in lymphoma cell line lysates. CD20 over expression lysate was used as the positive control. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/CD20-Overexpression-Lysate-Native-Western-Blot-NBL1-13312-img0003.jpg "Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Detection of CD20 in lymphoma cell line lysates. CD20 over expression lysate was used as the positive control. This image was submitted via customer Review.")
![Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD20.](http://images.novusbio.com/fullsize/CD20-Overexpression-Lysate-Native-Western-Blot-NBL1-13312-img0002.jpg)
![Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643] - Western blot analysis of extracts of various cell lines, using Insulin antibody (NBP3-15643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Western-Blot-NBP3-15643-img0005.jpg "Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643] - Western blot analysis of extracts of various cell lines, using Insulin antibody (NBP3-15643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Immunohistochemistry-Paraffin-NBP3-15643-img0001.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded mouse pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Immunohistochemistry-Paraffin-NBP3-15643-img0002.jpg)
![Immunohistochemistry: Insulin Antibody (4C3Y9) [NBP3-15643] -Confocal imaging of human pancreas using Insulin Rabbit mAb (at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15643_rabbit-insulin-mab-4c3y9-immunohistochemistry-23102024105444..jpg)
![Western Blot: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Analysis using CD158E1 mAb against human CD158E1 (AA: extra 206-340) recombinant protein. (Expected MW is 40.5 kDa)](http://images.novusbio.com/fullsize/KIR3DL1-Antibody-2C3B6-Western-Blot-NBP2-61687-img0004.jpg "Western Blot: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Analysis using CD158E1 mAb against human CD158E1 (AA: extra 206-340) recombinant protein. (Expected MW is 40.5 kDa)")
![Immunohistochemistry: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Analysis of paraffin-embedded cervical cancer tissues using CD158E1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/KIR3DL1-Antibody-2C3B6-Immunohistochemistry-NBP2-61687-img0003.jpg)
![Flow Cytometry: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Analysis of HL-60 cells using CD158E1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/KIR3DL1-Antibody-2C3B6-Flow-Cytometry-NBP2-61687-img0002.jpg)
![Western Blot: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Analysis using CD158E1 mAb against HEK293 (1) and CD158E1 (AA: extra 206-340)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/KIR3DL1-Antibody-2C3B6-Western-Blot-NBP2-61687-img0005.jpg)
![ELISA: KIR3DL1 Antibody (2C3B6) [NBP2-61687] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/KIR3DL1-Antibody-2C3B6-ELISA-NBP2-61687-img0001.jpg)
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0005.jpg "Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type III Collagen-UNLB (NBP1-26547; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.")
![Immunohistochemistry-Paraffin: Goat IgG Isotype Control [NB410-28088] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (NB410-28088; left) and Goat Anti-Type IV Collagen-UNLB (NBP1-26549 right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.](http://images.novusbio.com/fullsize/Goat-IgG-Isotype-Control-Immunohistochemistry-Paraffin-NB410-28088-img0003.jpg)
![Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-Western-Blot-NBP2-52422-img0003.jpg "Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)")
![Immunocytochemistry/Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of MCF-7 cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-Immunocytochemistry-Immunofluorescence-NBP2-52422-img0005.jpg)
![Flow Cytometry: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of Hela cells using BMP2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-Flow-Cytometry-NBP2-52422-img0008.jpg)
![Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis using BMP2 mAb against HEK293 (1) and BMP2 (AA: 283-396)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-Western-Blot-NBP2-52422-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Immunofluorescence analysis of Hela cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-Immunocytochemistry-Immunofluorescence-NBP2-52422-img0006.jpg)
![ELISA: BMP-2 Antibody (9E10G12) [NBP2-52422] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/BMP-2-Antibody-9E10G12-ELISA-NBP2-52422-img0007.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Western-Blot-NBP2-37328-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Immunocytochemistry-Immunofluorescence-NBP2-37328-img0004.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Flow-Cytometry-NBP2-37328-img0005.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Western-Blot-NBP2-37328-img0002.jpg)
![ELISA: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-ELISA-NBP2-37328-img0001.jpg)
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the PE conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0004.jpg "Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the PE conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.")
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - 1 ug/106 BALB/c splenocytes were incubated with either hamster IgG (as an isotype control) or purified hamster anti-mouse CD79, and then stained with mouse anti-hamster IgG (H+L)-FITC. Small lymphocytes were gated and analyzed on a flow cytometer](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0001.jpg)
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the Biotin conjugate of NBP1-28140. Multiple staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0002.jpg)
![Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the FITC conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD79B-Antibody-HM79-12-Flow-Cytometry-NBP1-28140-img0003.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0005.jpg "Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Immunocytochemistry-Immunofluorescence-NBP2-61863-img0003.jpg)
![Flow Cytometry: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0002.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0004.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0006.jpg)
![ELISA: HLA DRA Antibody (1C11A5) [NBP2-61863] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0001.jpg)
![SDS-Page: Rat IL-4 Protein [NBP2-35252]](http://images.novusbio.com/fullsize/Rat-IL-4-Protein-SDS-Page-NBP2-35252-img0001.jpg "SDS-Page: Rat IL-4 Protein [NBP2-35252]")
![Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.](http://images.novusbio.com/fullsize/Recombinant-Rat-IL-4-Protein-Bioactivity-NBP2-35252-img0002.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Western-Blot-NBP2-61726-img0004.jpg "Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)")
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis of cervical cancer tissues using CD1C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Immunohistochemistry-NBP2-61726-img0003.jpg)
![Flow Cytometry: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis of Ramos cells using CD1C mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Flow-Cytometry-NBP2-61726-img0002.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against HEK293 (1) and CD1C (AA: extra 18-302)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Western-Blot-NBP2-61726-img0005.jpg)
![ELISA: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-ELISA-NBP2-61726-img0001.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-278202415295599.jpg "Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32163) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-298202414115445.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32163) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-29202410234233.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Flow cytometric analysis of THP-1 (positive) and Jurkat (negative) cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32163, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-392024884626.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-278202415242671.jpg "Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29820241492652.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunocytochemistry analysis of SiHa cells labeling IL-7R alpha/CD127 with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29202410484826.jpg)
![Flow Cytometry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Flow cytometric analysis of K-562 cells labeling IL-7R alpha/CD127. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32122, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-392024884614.jpg)