| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21126 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08969 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70335 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41326-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43416-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81088-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-2005-0.002g | |||
| HRP | NB600-850-0.1mg | |||
![Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-Paraffin-NBP3-07898-img0002.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).")
![Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-NBP3-07898-img0001.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-2711202419381793.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241933343.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM , (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241928862.gif)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0005.jpg "Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0003.jpg)
![Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Western-Blot-NBP2-01814-img0004.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Hela cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0001.jpg)
![Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Jurkat cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Calpain-2-Antibody-1F4-Flow-Cytometry-NBP2-01814-img0002.jpg)
![Western Blot: ORAI3 Antibody (2H2G9) [NBP2-41326] - Analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ORAI3-Antibody-2H2G9-Western-Blot-NBP2-41326-img0003.jpg "Western Blot: ORAI3 Antibody (2H2G9) [NBP2-41326] - Analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody (2H2G9) [NBP2-41326] - Staining of ORAI3 in human spleen tissue with ORAI3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ORAI3-Antibody-2H2G9-Immunocytochemistry-Immunofluorescence-NBP2-41326-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody (2H2G9) - BSA Free [NBP2-41326] - Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41326_mouse-monoclonal-orai3-antibody-2h2g9-2711202419313221.jpg)
![Immunohistochemistry: ORAI3 Antibody (2H2G9) - BSA Free [NBP2-41326] - Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41326_mouse-monoclonal-orai3-antibody-2h2g9-2711202420392724.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0004.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0003.jpg)
![Western Blot: IL11RA Antibody [NBP1-62351] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/IL11RA-Antibody-Western-Blot-NBP1-62351-img0002.jpg "Western Blot: IL11RA Antibody [NBP1-62351] - Human Muscle lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: IL-11R alpha Antibody [NBP1-62351] - The effect of anti-human IL11Ralpha antibody combination treatment with doxorubicin on AN3CA xenograft tumour morphology in vivo. Representative photomicrographs of hematoxylin and eosin stained AN3CA subcutaneous tumour sections are shown in the top panel, arrows denote areas of tissue necrosis. AN3CA subcutaneous tumour sections were immunostained for pSTAT3, cleaved-caspase-3 (apoptosis marker) and Ki-67 (proliferation marker). Bars represent 100 um (H&E), 50 um (pSTAT3) and 200 um (caspase-3, Ki-67). Insets are negative controls. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/15187) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-11R-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-62351-img0003.jpg)
![Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Western-Blot-NBP3-15612-img0004.jpg "Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0001.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: BTK Antibody (2J9O8) [BTK] - Immunofluorescence analysis of paraffin-embedded mouse spleen using BTK Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15612_rabbit-btk-mab-2j9o8-52202517311037.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0002.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.")
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0001.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) - Chimeric [NBP2-81088] - Flow-cytometry using anti-CD44 antibody (Hermes-1) [NBP2-81088]. Human activated T lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Hermes-1 (NBP2-81088, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Chimeric-Flow-Cytometry-NBP2-81088-img0001.jpg "Flow Cytometry: CD44 Antibody (Hermes-1) - Chimeric [NBP2-81088] - Flow-cytometry using anti-CD44 antibody (Hermes-1) [NBP2-81088]. Human activated T lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Hermes-1 (NBP2-81088, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0010.jpg "Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0009.jpg)
![ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-ELISA-NBP1-77790-img0011.jpg)
![Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Immunoprecipitation-NBP2-43564-img0001.jpg "<b>Biological Strategies Validation. </b>Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-910202419593539.jpg)
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242014673.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242075915.jpg)
![Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (NBP2-43564) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-western-blot-261120251983622.jpg)