| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54473-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26866-25ul | |||
| Azide and BSA Free | NBP3-44288 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44739 | |||
| Azide and BSA Free | NBP2-34792-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64347SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70345 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0008.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunocytochemistry-Immunofluorescence-NBP2-53239-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0006.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Flow-Cytometry-NBP2-53239-img0004.jpg)
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0001.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0002.jpg)
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Siglec-2/CD22 Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Protein-Array-NBP2-53239-img0007.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Western-Blot-NBP1-51991-img0001.jpg "Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Flow-Cytometry-NBP1-51991-img0003.jpg)
![Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-Immunofluorescence-NBP1-51991-img0002.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023922510.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp1-51991_goat-polyclonal-blimp1-prdm1-antibody-imgenex-img-31243-1652023918332.jpg)
![Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Western-Blot-NBP2-37585-img0003.jpg "Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BLNK Antibody (5G9) [NBP2-37585] - Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Immunocytochemistry-Immunofluorescence-NBP2-37585-img0004.jpg)
![Immunohistochemistry-Paraffin: BLNK Antibody (5G9) [NBP2-37585] - Analysis of human cervical cancer tissues using BLNK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Immunohistochemistry-NBP2-37585-img0006.jpg)
![Flow Cytometry: BLNK Antibody (5G9) [NBP2-37585] - Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Flow-Cytometry-NBP2-37585-img0005.jpg)
![Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-Western-Blot-NBP2-37585-img0002.jpg)
![ELISA: BLNK Antibody (5G9) [NBP2-37585] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BLNK-Antibody-5G9-ELISA-NBP2-37585-img0001.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Western Blot: CD79A Antibody (SPM550) [NBP2-32834] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (SPM550).](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM550-Western-Blot-NBP2-32834-img0004.jpg "Western Blot: CD79A Antibody (SPM550) [NBP2-32834] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (SPM550).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (SPM550) [NBP2-32834] - Immunofluorescence Analysis of PFA-fixed Raji cells labeling CD79a with CD79A Antibody (SPM550) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM550-Immunocytochemistry-Immunofluorescence-NBP2-32834-img0002.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (SPM550) [NBP2-32834] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (SPM550)](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM550-Immunohistochemistry-Paraffin-NBP2-32834-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (SPM550) [NBP2-32834] - Formalin-fixed, paraffin-embedded tonsil (10X) stained with CD79a MAb (SPM550).](http://images.novusbio.com/fullsize/CD79A-Antibody-SPM550-Immunohistochemistry-Paraffin-NBP2-32834-img0001.jpg)
![Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Western-Blot-NBP2-45868-img0005.jpg "Western Blot: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7.")
![Immunohistochemistry-Paraffin: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Paraffin-embedded Carcinoma of Human prostate tissue using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-Paraffin-NBP2-45868-img0006.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0001.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0002.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0003.jpg)
![Immunohistochemistry: CRACC/SLAMF7 Antibody (OTI1F1) [NBP2-45868] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-OTI1F1-Immunohistochemistry-NBP2-45868-img0004.jpg)
![Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.](http://images.novusbio.com/fullsize/CD79A-Antibody-HM57-Immunohistochemistry-NB100-64347-img0005.jpg "Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.")
![Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/CD79A-Antibody-HM57-Flow-Cytometry-NB100-64347-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.](http://images.novusbio.com/fullsize/CD79A-Antibody-HM57-Immunohistochemistry-Paraffin-NB100-64347-img0002.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c .](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Western-Blot-NBP2-46123-img0007.jpg "Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c .")
![Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD1C.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunofluorescence-NBP2-46123-img0009.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunohistochemistry-NBP2-46123-img0006.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Jurkat extracts (10ug).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Western-Blot-NBP2-46123-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunofluorescence-NBP2-46123-img0002.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunohistochemistry-NBP2-46123-img0003.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunohistochemistry-NBP2-46123-img0004.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Immunohistochemistry-NBP2-46123-img0005.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c .](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Western-Blot-NBP2-70345-img0007.jpg "Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c .")
![Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70345-img0008.jpg)
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD1C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70345-img0005.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Analysis of extracts (10ug) from 1 cell lines by using anti-CD1C monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Western-Blot-NBP2-70345-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70345-img0006.jpg)
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD1C mouse monoclonal antibody](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70345-img0002.jpg)
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CD1C mouse.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70345-img0003.jpg)
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (OTI2F4) - Azide and BSA Free [NBP2-70345] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD1C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-OTI2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-70345-img0004.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)