| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90437-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12003-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81119-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81100-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75899SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52657-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
![Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Immunohistochemistry-Paraffin-NBP2-90437-img0003.jpg "Immunohistochemistry-Paraffin: SR-AI/MSR Antibody (004) [NBP2-90437] - Immunochemical staining of mouse SR-AI/MSR in mouse liver with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).")
![Flow Cytometry: SR-AI/MSR Antibody (004) [NBP2-90437] - Flow cytometric analysis of Mouse SR-AI/MSR expression on Raw264.7 cells. Cells were stained with purified anti-Mouse SR-AI/MSR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-004-Flow-Cytometry-NBP2-90437-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody (004) [NBP2-90437] - Confocal immunofluorescence analysis of Mouse SR-AI/MSR1 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90437 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/nbp2-90437_rabbit-monoclonal-sr-ai-msr-antibody-004-235202511444470.png)
![Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - THP-1 cell lysate, Antibody Titration: 1.0ug/ml](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Western-Blot-NBP1-59062-img0004.jpg "Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - THP-1 cell lysate, Antibody Titration: 1.0ug/ml")
![Immunohistochemistry-Paraffin: CYBB/NOX2 Antibody [NBP1-59062] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Immunohistochemistry-Paraffin-NBP1-59062-img0003.jpg)
![Western Blot: CYBB/NOX2 Antibody [NBP1-59062] - MCF7 tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Western-Blot-NBP1-59062-img0001.jpg)
![Immunohistochemistry-Paraffin: CYBB/NOX2 Antibody [NBP1-59062] - Human Spleen](http://images.novusbio.com/fullsize/CYBB-NOX2-Antibody-Immunohistochemistry-Paraffin-NBP1-59062-img0005.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-278202415212467.jpg "Western Blot: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 1 minute 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32164) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-298202414161818.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C2-R) [NBP3-32164] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32164) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32164) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32164_mouse-cd68-sr-d1-mab-a3c2-r-29202410234268.jpg)
![Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-278202415212462.jpg "Western Blot: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: Human brain tissue lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32133) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32133) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-298202413585577.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-49) [NBP3-32133] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32133_rabbit-cd200-ox2-mab-psh01-49-392024884632.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0003.jpg "Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.")
![Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-NBP2-31130-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)
![Western Blot: CXCR1/IL-8RA Antibody (SE2) - Chimeric [NBP3-12003] - Human lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (NBP3-12003) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CXCR1-IL-8RA-Antibody-SE2-Chimeric-Western-Blot-NBP3-12003-img0001.jpg "Western Blot: CXCR1/IL-8RA Antibody (SE2) - Chimeric [NBP3-12003] - Human lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (NBP3-12003) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CXCR1/IL-8RA Antibody (SE2) - Chimeric [NBP3-12003] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of SE2 (NBP3-12003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12003, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CXCR1-IL-8RA-Antibody-SE2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12003-img0002.jpg)
![Flow Cytometry: CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free [NBP3-12003] - Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12003 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12003_rabbit-monoclonal-cxcr1-il-8ra-antibody-se2-chimeric-74202522141219.png)
![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-IDEC-114-Galiximab-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81100-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Western-Blot-NBP2-75899-img0001.jpg "Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.")
![Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Chimeric-Flow-Cytometry-NBP2-52657-img0001.jpg "Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)