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![Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-278202415242688.jpg "Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32097) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-29820241411543.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0002.jpg "Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).")
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0001.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-MEM-188-Flow-Cytometry-NBP3-09126-img0001.jpg "Flow Cytometry: NCAM-1/CD56 Antibody (MEM-188) [NBP3-09126] - Analysis (surface staining) of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.")
![Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57520-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.")
![Immunohistochemistry-Paraffin: TDO2 Antibody (C1) [NBP3-41732] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41732; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41732_mouse-tdo2-mab-c1-61220242151876.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C1) [NBP3-41732] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41732; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TDO2 Antibody (C1) [NBP3-41732] - Sample: Rat Liver lysate; Primary Ab: 3ug/ml NBP3-41732; Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41732_mouse-tdo2-mab-c1-612202420484722.jpg)
![Western Blot: LIFR alpha Antibody [NBP2-32070] - LIFR Antibody [NBP2-32070] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-LIFR antibody NBP2-32070 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LIFR-alpha-Antibody-Western-Blot-NBP2-32070-img0002.jpg "Western Blot: LIFR alpha Antibody [NBP2-32070] - LIFR Antibody [NBP2-32070] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-LIFR antibody NBP2-32070 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")