| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61791-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61893-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61680-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2623-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11969SS | |||
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0004.jpg "Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Immunocytochemistry-Immunofluorescence-NBP2-61791-img0003.jpg)
![Flow Cytometry: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0002.jpg)
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against HEK293 (1) and PLXNA1 (AA: 1100-1200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0005.jpg)
![ELISA: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0001.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0004.jpg "Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Immunocytochemistry-Immunofluorescence-NBP2-61893-img0003.jpg)
![Flow Cytometry: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Flow-Cytometry-NBP2-61893-img0002.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against HEK293 (1) and SYK (AA: 217-356)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0005.jpg)
![ELISA: SYK Antibody (2G11E6) [NBP2-61893] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-ELISA-NBP2-61893-img0001.jpg)
![Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mAb against HEK293 (1) and CD197 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-Western-Blot-NBP2-61680-img0004.jpg "Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mAb against HEK293 (1) and CD197 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis of K562 cells using CCR7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-Flow-Cytometry-NBP2-61680-img0006.jpg)
![Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mAb against human CD197 (AA: extra mix) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-Western-Blot-NBP2-61680-img0003.jpg)
![Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-Western-Blot-NBP2-61680-img0005.jpg)
![Flow Cytometry: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis of HL-60 cells using CCR7 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-Flow-Cytometry-NBP2-61680-img0002.jpg)
![ELISA: CCR7 Antibody (4B7B8) [NBP2-61680] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CCR7-Antibody-4B7B8-ELISA-NBP2-61680-img0001.jpg)
![Immunohistochemistry-Paraffin: proBNP Antibody (3A6F7C7) [NBP1-47509] - Analysis of human normal myocardium, showing cytoplasmic localization using BNP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/proBNP-Antibody-3A6F7C7-Immunohistochemistry-Paraffin-NBP1-47509-img0002.jpg "Immunohistochemistry-Paraffin: proBNP Antibody (3A6F7C7) [NBP1-47509] - Analysis of human normal myocardium, showing cytoplasmic localization using BNP3 mouse mAb with DAB staining.")
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human liver](http://images.novusbio.com/fullsize/FPRL1-FPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-14323-img0005.jpg "Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human liver")
![Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human spleen, neutrophils, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/FPRL1-FPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-14323-img0001.jpg)
![Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lymph node, Hodgkins lymphoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/FPRL1-FPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-14323-img0002.jpg)
![Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon, neutrophils in crypt abscess, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/FPRL1-FPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-14323-img0003.jpg)
![Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of formalin-fixed, paraffin-embedded human spleen](http://images.novusbio.com/fullsize/FPRL1-FPR2-Antibody-Immunohistochemistry-Paraffin-NBP3-14323-img0004.jpg)
![Immunohistochemistry: FPRL1/FPR2 Antibody [NBP3-14323] - Immunohistochemistry of breast, adenocarcinoma,](http://images.novusbio.com/fullsize/FPRL1-FPR2 Antibody-Immunohistochemistry-NBP3-14323-img0006.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)
![Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-278202415295563.jpg "Western Blot: KIF3A Antibody (JE31-39) [NBP3-32501] - Western blot analysis of KIF3A on different lysates with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (40 ug/Lane) Lane 3: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32501) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32501) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-298202413585569.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (JE31-39) [NBP3-32501] - Immunocytochemistry analysis of NIH/3T3 cells labeling KIF3A with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIF3A antibody (NBP3-32501) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32501_rabbit-kif3a-mab-je31-39-29202410334094.jpg)
![Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-EM-05-Flow-Cell-Surface-NBP1-44763-img0002.jpg "Flow (Cell Surface): CD45 Antibody (EM-05) [NBP1-44763] - Analysis using the Allophycocyanin conjugate of CD45 antibody (EM-05) [NBP1-44763]. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)