| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93944-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37276-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2623-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61791-0.025ml | |||
![Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TNFAIP1-Antibody-Western-Blot-NBP2-82039-img0001.jpg "Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.")
![Immunohistochemistry: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunohistochemistry of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-271120241935386.jpg)
![Immunocytochemistry/ Immunofluorescence: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunofluorescence of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 20 ug/ml.Red: TNFAIP1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-2711202420285833.gif)
![Western Blot: PSG1 Antibody [NBP2-93944] - Western blot analysis of extracts of various cell lines, using PSG1 antibody (NBP2-93944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PSG1 Antibody-Western Blot-NBP2-93944-img0005.jpg "Western Blot: PSG1 Antibody [NBP2-93944] - Western blot analysis of extracts of various cell lines, using PSG1 antibody (NBP2-93944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: PSG1 Antibody [NBP2-93944] - Analysis of human placenta cells using PSG1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PSG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93944-img0001.jpg)
![Immunohistochemistry-Paraffin: PSG1 Antibody [NBP2-93944] - Paraffin-embedded human stomach using PSG1 .](http://images.novusbio.com/fullsize/PSG1-Antibody-Immunohistochemistry-Paraffin-NBP2-93944-img0003.jpg)
![Immunohistochemistry-Paraffin: PSG1 Antibody [NBP2-93944] - Paraffin-embedded human colon carcinoma using PSG1 .](http://images.novusbio.com/fullsize/PSG1-Antibody-Immunohistochemistry-Paraffin-NBP2-93944-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0003.jpg)
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-ELISA-NBP2-37276-img0001.jpg)
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0004.jpg "Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Immunocytochemistry-Immunofluorescence-NBP2-61791-img0003.jpg)
![Flow Cytometry: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0002.jpg)
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against HEK293 (1) and PLXNA1 (AA: 1100-1200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0005.jpg)
![ELISA: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0001.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0002.jpg "Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.")
![Immunocytochemistry/Immunofluorescence: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71420-img0004.jpg)
![Immunohistochemistry: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71420-img0003.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of extracts (35ug) from 9 different cell lines by using CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0002.jpg "Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).")
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0001.jpg)
![Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-28820241423310.jpg "Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg)
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)
![Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-27820241631626.jpg "Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-298202414161875.jpg)
![Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunocytochemistry analysis of Raji cells labeling Neprilysin/CD10 with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-29202410484874.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09101-img0002.jpg "Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (15-2) - Low Endotoxin, Azide and BSA Free [NBP3-09101] - Surface staining pattern of human human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 ug/ml), GAM PE.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-15-2-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09101-img0001.jpg)