| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90577-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90097-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81042-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81052-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81054-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
![Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-278202416331267.jpg "Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD3 Antibody (PD01-43) [NBP3-32140] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-29820241492648.jpg)
![Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD48-SLAMF2-Antibody-341-Flow-Cytometry-NBP2-90577-img0001.jpg "Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Immunofluorescence staining of Mouse CD48/SLAMF2 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90577 antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/nbp2-90577_rabbit-monoclonal-cd48-slamf2-antibody-341-235202514331563.png)
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0002.jpg "Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human appendix with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0001.jpg)
![Western Blot: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Western blot analysis of CD48/SLAMF2 on different lysates with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27.7 kDa Observed band size: 40 kDa Exposure time: 80 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32157) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32157_rabbit-cd48-slamf2-mab-je37-98-2782024163160.jpg "Western Blot: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Western blot analysis of CD48/SLAMF2 on different lysates with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27.7 kDa Observed band size: 40 kDa Exposure time: 80 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32157) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32157_rabbit-cd48-slamf2-mab-je37-98-29820241435245.jpg)
![Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-27820241631671.jpg "Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-29820241433368.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-2982024143520.jpg "Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Flow cytometric analysis of HEK-293 cells labeling B7-H3/CD276. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32138, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-29202416453234.jpg)
![Immunocytochemistry/Immunofluorescence: CD2 Antibody (YTH 655) [NBP2-81042] - Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YTH 655 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane, and some cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81402, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD2-Antibody-YTH-655-Immunocytochemistry-Immunofluorescence-NBP2-81042-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD2 Antibody (YTH 655) [NBP2-81042] - Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YTH 655 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane, and some cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81402, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23396_mouse-integrin-beta-4-cd104-mab-m126-19920231856926.jpg "Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23396_mouse-integrin-beta-4-cd104-mab-m126-19920231992811.jpg)
![Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-YNB46-1-8-Campath-9H-Chimeric-Flow-Cytometry-NBP2-52658-img0002.jpg "Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)