| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| CoraFluor 1 | NBP2-34703CL1 | |||
| FITC | NBP2-34703F | |||
| Janelia Fluor 525 | NBP2-34703JF525 | |||
| Janelia Fluor 669 | NBP2-34703JF669 | |||
| mFluor Violet 450 SE | NBP2-34703MFV450 | |||
| mFluor Violet 500 SE | NBP2-34703MFV500 | |||
| mFluor Violet 610 SE | NBP2-34703MFV610 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34703-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78782SS | |||
| Azide and BSA Free | NBP2-80714 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72117 | |||
![Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (P/T2) [NBP2-34302] - Immunofluorescence staining of HePG2 cells using TNF-alphaMouse Monoclonal Antibody (P/T2) followed by goat anti-mouse IgG-CF488. Counterstained with RedDot.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunocytochemistry-Immunofluorescence-NBP2-34302-img0003.jpg "Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody (P/T2) [NBP2-34302] - Immunofluorescence staining of HePG2 cells using TNF-alphaMouse Monoclonal Antibody (P/T2) followed by goat anti-mouse IgG-CF488. Counterstained with RedDot.")
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (P/T2) [NBP2-34302] - Formalin-fixed, paraffin-embedded human Pancreas stained with TNF-alpha Antibody (P/T2).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunohistochemistry-Paraffin-NBP2-34302-img0002.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (P/T2) [NBP2-34302] - Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (P/T2).](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-P-T2-Immunohistochemistry-Paraffin-NBP2-34302-img0001.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0011.jpg "Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in NIH/3T3.")
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4 Analysis of NADK expression in HeLa.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0008.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in HepG2.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0009.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - NADK monoclonal antibody (M01), clone 5F4. Analysis of NADK expression in Raw 264.7.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0010.jpg)
![Western Blot: NADK Antibody (5F4) [H00065220-M01] - Analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-Western-Blot-H00065220-M01-img0012.jpg)
![ELISA: NADK Antibody (5F4) [H00065220-M01] - Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NADK-Antibody-5F4-ELISA-H00065220-M01-img0013.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249351317.jpg "Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249425493.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-212024941472.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249383189.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (HL2314) - Azide and BSA Free [NBP3-25723] - TLR3 antibody [HL2314] detects TLR3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. TLR3 stained by TLR3 antibody [HL2314] (NBP3-25723) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25723_rabbit-tlr3-mab-hl2314-azide-and-bsa-free-2120249355579.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Western-Blot-NBP2-78782-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - Western blot shows lysates of PC3 human prostate cancer cell line, HEK293T human embryonic kidney cell line, and NIH3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EGLN1/PHD2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445D) [NBP2-78782] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-78782) at 3 ug/mL for 1 hour at room temperature followed by incubation with an anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445D-Immunohistochemistry-Paraffin-NBP2-78782-img0002.jpg)
![Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-78132-img0002.jpg "Western Blot: IL-6 Antibody [NBP2-78132] - Mouse IL-6 recombinant protein was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-IL-6 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-78132] - IHC analysis of a formalin fixed paraffin embedded tissue section of human appendix using 1:200 dilution of IL-6 antibody (NBP2-78132). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated membrane staining of glandular cells.](http://images.novusbio.com/fullsize/IL-6-Antibody-Immunohistochemistry-Paraffin-NBP2-78132-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.")
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0007.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD38 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) [NBP2-46145] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Western-Blot-NBP2-46145-img0002.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0004.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0005.jpg)
![Immunohistochemistry-Paraffin: CD38 Antibody (OTI1C9) [NBP2-46145] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Immunohistochemistry-Paraffin-NBP2-46145-img0006.jpg)
![Western Blot: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE-2.](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Western-Blot-NBP2-46490-img0005.jpg "Western Blot: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE-2.")
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0004.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0001.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0002.jpg)
![Immunohistochemistry: ACE-2 Antibody (OTI4D2) [NBP2-46490] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Immunohistochemistry-NBP2-46490-img0003.jpg)
![Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Western-Blot-NB100-65672-img0002.jpg "Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate")
![Immunohistochemistry: CD19 Antibody (LE-CD19) [NB100-65672] - Mouse anti human CD19 staining formalin-fixed, paraffin-embedded human tonsil.](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Immunohistochemistry-NB100-65672-img0001.jpg)
![Western Blot: ATF2 Antibody [NB100-835] - Human primary astrocyte lysates probed with ATF2 antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATF2-Antibody-Western-Blot-NB100-835-img0007.jpg "Western Blot: ATF2 Antibody [NB100-835] - Human primary astrocyte lysates probed with ATF2 antibody. WB image submitted by a verified customer review.")
![Western Blot: ATF2 Antibody [NB100-835] - Staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ATF2-Antibody-Western-Blot-NB100-835-img0006.jpg)
![Western Blot: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Analysis of extracts of various cell lines, using Syntenin 1 Rabbit mAb (NBP3-16603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Western-Blot-NBP3-16603-img0005.jpg "Western Blot: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Analysis of extracts of various cell lines, using Syntenin 1 Rabbit mAb (NBP3-16603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Mouse brain using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0003.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Rat brain using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0001.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody (3J5O6) [NBP3-16603] - Human colon using Syntenin 1 Rabbit mAb (NBP3-16603) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-3J5O6-Immunohistochemistry-Paraffin-NBP3-16603-img0002.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (3J5O6) [Syntenin 1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Syntenin 1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16603_rabbit-syntenin-1-mab-3j5o6-52202517152745.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)