| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54548-100ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0207A488 | |||
| Alexa Fluor 546 | DDX0207A546 | |||
| Alexa Fluor 647 | DDX0207A647 | |||
| Biotin | DDX0207B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80035-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80038-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90819-50ul | |||
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Knockout-Validated-NBP2-53318-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) [NBP2-53318] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) [NBP2-53318] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Immunohistochemistry-Paraffin-NBP2-53318-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) [NBP2-53318] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Flow-Cytometry-NBP2-53318-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunocytochemistry-Immunofluorescence-DDX0207-img0007.jpg "Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN")
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Tonsil](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunohistochemistry-Paraffin-DDX0207-img0008.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis of Alexa Fluor (R) 647 conjugate of DDX0207. FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0011.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0207. A) Staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0009.jpg)
![Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Western-Blot-NBP2-80035-img0003.jpg "Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).")
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) [NBP2-80035] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80035) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Immunohistochemistry-Paraffin-NBP2-80035-img0006.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0005.jpg)
![Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC18F-Flow-Cytometry-NBP2-80035-img0001.jpg)
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0003.jpg "Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).")
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 0.5 ug/mL sheep Fc-tagged recombinant human Spike S1 is shown to bind ACE-2 (black dotted line) detected by Donkey anti-Sheep APC Secondary Antibody (Catalog # F0127). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0004.jpg)
![Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Western-Blot-NBP2-80038-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0007.jpg)
![Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Flow-Cytometry-NBP2-80038-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0006.jpg)
![Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0001.jpg)
![Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Western-Blot-NBP2-67605-img0006.jpg "Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0002.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0001.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0003.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0004.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0005.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0015.jpg "Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0013.jpg)
![Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/Roquin-Antibody-Western-Blot-NB100-655-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18057-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD4 antibody (NBP3-18057). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP3-18057-img0001.jpg)
![Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP3-18057-img0003.jpg)
![Immunohistochemistry-Paraffin: Rabbit CD4 pAb [NBP3-18057] - Immunofluorescence staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP3-18057AF647) in 3% BSA and incubated over night at 4 °C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-18057_rabbit-cd4-pab-immunohistochemistry-paraffin-49202411280..jpg)
![Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Neprilysin/CD10 on different lysates with Rabbit anti-Neprilysin/CD10 antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 ug/Lane) Lane 2: Mouse small intestine tissue lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Lane 4: Mouse placenta tissue lysate (20 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Western-Blot-NBP2-67717-img0009.jpg "Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Neprilysin/CD10 on different lysates with Rabbit anti-Neprilysin/CD10 antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 ug/Lane) Lane 2: Mouse small intestine tissue lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Lane 4: Mouse placenta tissue lysate (20 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of 293 cells labeling CD10 with Rabbit anti-Neprilysin/CD10 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD10 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0010.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0013.jpg)
![Flow Cytometry: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Flow-Cytometry-NBP2-67717-img0001.jpg)
![Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of CD10 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Western-Blot-NBP2-67717-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0004.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0005.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0006.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0007.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0011.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0012.jpg)
![Immunohistochemistry-Paraffin: CXCR6 Antibody [NLS1102] - Anti-CXCR6 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR6-Antibody-Immunohistochemistry-Paraffin-NLS1102-img0005.jpg "Immunohistochemistry-Paraffin: CXCR6 Antibody [NLS1102] - Anti-CXCR6 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CXCR6 Antibody [NLS1102] - Analysis of anti-CXCR6 antibody with human lung, adenocarcinoma.](http://images.novusbio.com/fullsize/CXCR6-Antibody-Immunohistochemistry-Paraffin-NLS1102-img0003.jpg)
![Immunohistochemistry-Paraffin: CXCR6 Antibody [NLS1102] - Analysis of anti-CXCR6 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/CXCR6-Antibody-Immunohistochemistry-Paraffin-NLS1102-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCR6 Antibody [NLS1102] - Analysis of anti-CXCR6 antibody with tonsil, interfollicular zone at 6.8 ug/ ml.](http://images.novusbio.com/fullsize/CXCR6-Antibody-Immunohistochemistry-Paraffin-NLS1102-img0001.jpg)
![Immunohistochemistry-Paraffin: CXCR6 Antibody - BSA Free [NLS1102] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Kidney tissue](http://images.novusbio.com/fullsize/nls1102_rabbit-cxcr6-pab-bsa-free-10920241103113.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0001.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).")
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Knockout-Validated-NBP2-54548-img0005.jpg)