| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15592-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50447-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-37721 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20094-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33985-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35245-20ul | |||
![Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NAP1 Antibody [NBP1-26592] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. TAB3 was also immunoprecipitated by rabbit anti-TAB3 antibody NBP1-26593.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0001.jpg)
![Immunoprecipitation: NAP1 Antibody [NBP1-26592] - Detection of human TAB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 (lot NBP1-26592-2) used for IP at 6 ug per reaction. TAB3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26592-1). For blotting immunoprecipitated TAB3, NBP1-26592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunoprecipitation-NBP1-26592-img0004.jpg)
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-61220242151820.jpg "Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Orosomucoid 2 Antibody (D7) [NBP3-41442] - DAB staining on IHC-P; Sample: Human Prostate cancer Tissue; Primary Ab: 30ug/ml NBP3-41442; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202421351278.jpg)
![Western Blot: Orosomucoid 2 Antibody (D7) [NBP3-41442] - Sample: Lane1: Human Serum; Lane2: Rat Liver lysate; Lane3: Rat Pancreas lysate; Lane4: Rat Bladder lysate Primary Ab: 3ug/ml NBP3-41442; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41442_mouse-orosomucoid-2-mab-d7-612202420484723.jpg)
![Western Blot: ATF2 Antibody [NB100-835] - Human primary astrocyte lysates probed with ATF2 antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATF2-Antibody-Western-Blot-NB100-835-img0007.jpg "Western Blot: ATF2 Antibody [NB100-835] - Human primary astrocyte lysates probed with ATF2 antibody. WB image submitted by a verified customer review.")
![Western Blot: ATF2 Antibody [NB100-835] - Staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ATF2-Antibody-Western-Blot-NB100-835-img0006.jpg)
![Western Blot: NAP1 Antibody [NBP3-15592] - Western blot analysis of extracts of various cell lines, using NAP1 pAb (NBP3-15592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP3-15592-img0002.jpg "Western Blot: NAP1 Antibody [NBP3-15592] - Western blot analysis of extracts of various cell lines, using NAP1 pAb (NBP3-15592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: NAP1 Antibody [NBP3-15592] - Immunohistochemistry of paraffin-embedded human liver cancer using NAP1 Rabbit pAb (NBP3-15592) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunohistochemistry-Paraffin-NBP3-15592-img0001.jpg)
![Western Blot: Myosin Heavy Chain 3 Antibody [NBP2-81968] - Analysis of Myosin Heavy Chain 3 in fetal human skeletal muscle lysate with Myosin Heavy Chain 3 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Myosin-Heavy-Chain-3-Antibody-Western-Blot-NBP2-81968-img0001.jpg "Western Blot: Myosin Heavy Chain 3 Antibody [NBP2-81968] - Analysis of Myosin Heavy Chain 3 in fetal human skeletal muscle lysate with Myosin Heavy Chain 3 antibody at 1 ug/ml.")
![Immunohistochemistry: Myosin Heavy Chain 3 Antibody [NBP2-81968] - Analysis of Myosin Heavy Chain 3 in mouse skeletal muscle tissue with Myosin Heavy Chain 3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Myosin-Heavy-Chain-3-Antibody-Immunohistochemistry-NBP2-81968-img0002.jpg)
![Western Blot: Orosomucoid 2 Antibody [NBP3-32680] - Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 24 kDa Observed band size: 54 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32680) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-278202415242661.jpg "Western Blot: Orosomucoid 2 Antibody [NBP3-32680] - Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LO2 cell lysate Lane 3: Huh7 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 24 kDa Observed band size: 54 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32680) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Orosomucoid 2 Antibody [NBP3-32680] - Immunocytochemistry analysis of HepG2 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (NBP3-32680) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-292024111487.jpg)
![Flow Cytometry: Orosomucoid 2 Antibody [NBP3-32680] - Flow cytometric analysis of HepG2 cells labeling Orosomucoid 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32680, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32680_rabbit-orosomucoid-2-pab-29202416372170.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free [NB500-309] - Surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/nb500-309_mouse-monoclonal-integrin-alpha-l-cd11a-antibody-mem-83-1772024100321.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free [NB500-309] - Surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/nb500-309_mouse-monoclonal-integrin-alpha-l-cd11a-antibody-mem-83-17720241003221.jpg)
![Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924252.png "Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-16D-6B9 (NBP3-20094) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20094, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924253.png)
![SDS-Page: Recombinant Human TIA1 Protein [H00007072-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007072-P01-1.jpg "SDS-Page: Recombinant Human TIA1 Protein [H00007072-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: NAP1 Antibody [NBP2-33985] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33985-img0006.jpg "Immunocytochemistry/Immunofluorescence: NAP1 Antibody [NBP2-33985] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.")
![Immunohistochemistry-Paraffin: NAP1 Antibody [NBP2-33985] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33985-img0002.jpg)
![Western Blot: TIA1 Antibody [NBP3-35245] - Western blot analysis of lysates from Jurkat cells, using TIA1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35245_rabbit-tia1-pab-1012202411392936.jpg "Western Blot: TIA1 Antibody [NBP3-35245] - Western blot analysis of lysates from Jurkat cells, using TIA1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: TIA1 Antibody [NBP3-35245] - Immunofluorescence analysis of HepG2 cells using TIA1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35245_rabbit-tia1-pab-31120251704771.jpg%3Ft=1698885529)