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![Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909] - Immunochemical staining of human CEACAM3/CD66d in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CEACAM3-CD66d-Antibody-Immunohistochemistry-Paraffin-NBP2-99909-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM3/CD66d Antibody [NBP2-99909] - Immunochemical staining of human CEACAM3/CD66d in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32119_rabbit-semaphorin-4d-cd100-mab-je63-21-278202415242698.jpg "Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Sandwich ELISA: Semaphorin 4D/CD100 Antibody Pair [HRP] [NBP2-79597] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Semaphorin-4D-CD100-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79597-img0001.jpg "Sandwich ELISA: Semaphorin 4D/CD100 Antibody Pair [HRP] [NBP2-79597] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666] - Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Semaphorin-4D-CD100-Antibody-010-Flow-Cytometry-NBP2-90666-img0002.jpg "Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666] - Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")