| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87184-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44029 | |||
| BSA Free | NBP2-52928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79916 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86555-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38155-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79919 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44921 | |||
| Azide and BSA Free | NBP2-34583-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11404 | |||
![Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis using Anti-CRP antibody NBP1-87184 (A) shows similar pattern to independent antibody NBP1-87183 (B).](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Western-Blot-NBP1-87184-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis using Anti-CRP antibody NBP1-87184 (A) shows similar pattern to independent antibody NBP1-87183 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human tonsil shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0011.jpg)
![Western Blot: C-Reactive Protein/CRP Antibody [NBP1-87184] - Analysis in human plasma.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Western-Blot-NBP1-87184-img0005.jpg)
![Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human duodenum shows moderate positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0009.jpg)
![Immunohistochemistry-Paraffin: C-Reactive Protein/CRP Antibody [NBP1-87184] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/C-Reactive-Protein-CRP-Antibody-Immunohistochemistry-Paraffin-NBP1-87184-img0010.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (CL0395) [NBP2-52928] - Staining in human tonsil and pancreas tissues. Corresponding CD4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD4-Antibody-CL0395-Immunohistochemistry-Paraffin-NBP2-52928-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD4 Antibody (CL0395) [NBP2-52928] - Staining in human tonsil and pancreas tissues. Corresponding CD4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD4 Antibody (CL0395) [NBP2-52928] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T , Lane 3: CD4 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/CD4-Antibody-CL0395-Western-Blot-NBP2-52928-img0004.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (CL0395) [NBP2-52928] - Staining of human tonsil shows moderate membranous and cytoplasmic positivity, mainly in non - germinal center cells.](http://images.novusbio.com/fullsize/CD4-Antibody-CL0395-Immunohistochemistry-Paraffin-NBP2-52928-img0007.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (CL0395) [NBP2-52928] - Staining of human small intestine shows moderate membranous and cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD4-Antibody-CL0395-Immunohistochemistry-Paraffin-NBP2-52928-img0008.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (CL0395) [NBP2-52928] - Staining of human pancreas shows no positivity in either islets of Langerhans or exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD4-Antibody-CL0395-Immunohistochemistry-Paraffin-NBP2-52928-img0009.jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Western Blot Analysis of PC-3 cell lysate using Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Western-Blot-NBP2-79782-img0004.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Western Blot Analysis of PC-3 cell lysate using Complement Component C1qA Antibody (C1QA/2953).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Immunohistochemistry-Paraffin-NBP2-79782-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Immunohistochemistry-Paraffin-NBP2-79782-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement Component C1qA Antibody (C1QA/2953). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Protein-Array-NBP2-79782-img0003.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining of human placenta shows moderate to strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Semaphorin-7A-Antibody-Immunohistochemistry-Paraffin-NBP1-86555-img0009.jpg "Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining of human placenta shows moderate to strong membranous positivity in trophoblastic cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining in human testis and skeletal muscle tissues.. Corresponding SEMA7A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Semaphorin-7A-Antibody-Immunohistochemistry-Paraffin-NBP1-86555-img0005.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining of human cerebral cortex shows moderate to strong membranous positivity in neurons.](http://images.novusbio.com/fullsize/Semaphorin-7A-Antibody-Immunohistochemistry-Paraffin-NBP1-86555-img0006.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Semaphorin-7A-Antibody-Immunohistochemistry-Paraffin-NBP1-86555-img0007.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-86555] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Semaphorin-7A-Antibody-Immunohistochemistry-Paraffin-NBP1-86555-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody [NBP2-38155] - Immunofluorescent staining of human cell line A549 shows localization to cytosol.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38155-img0003.jpg "Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody [NBP2-38155] - Immunofluorescent staining of human cell line A549 shows localization to cytosol.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining in human lymph node and cerebral cortex tissues using anti-PSMB10 antibody. Corresponding PSMB10 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0006.jpg)
![Immunohistochemistry: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human tonsil shows nuclear positivity in non germinal center.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-NBP2-38155-img0002.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0004.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP2-38155] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38155-img0005.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Western-Blot-NBP3-14749-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0002.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0001.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]- Rabbit anti-CD127/IL-7R alpha recombinantmonoclonal antibody [BLR177J] Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14749_rabbit-il-7r-alpha-cd127-mab-immunohistochemistry-paraffin-1012024122450..jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Western Blot Analysis of human PC3 cell lysate using Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Western-Blot-NBP2-79785-img0004.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Western Blot Analysis of human PC3 cell lysate using Complement Component C1qA Antibody (C1QA/2954).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Immunohistochemistry-Paraffin-NBP2-79785-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Formalin-fixed, paraffin-embedded human Kidney stained with Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Immunohistochemistry-Paraffin-NBP2-79785-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement Component C1qA Antibody (C1QA/2954). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Protein-Array-NBP2-79785-img0003.jpg)
![Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Immunoprecipitation-NBP2-43564-img0001.jpg "<b>Biological Strategies Validation. </b>Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-910202419593539.jpg)
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242014673.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242075915.jpg)
![Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (NBP2-43564) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-western-blot-261120251983622.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - Formalin-fixed paraffin-embedded human tonsil stained with CD5 Monoclonal antibody (C5/473+CD5/54/F6)](http://images.novusbio.com/fullsize/CD5-Antibody-C5-473-+-CD5-54-F6-Immunohistochemistry-Paraffin-NBP2-34316-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - Formalin-fixed paraffin-embedded human tonsil stained with CD5 Monoclonal antibody (C5/473+CD5/54/F6)")
![Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - CD5 CODEX stain of human tonsil tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34316_mouse-monoclonal-cd5-antibody-c5-473-cd5-54-f6-1772024936264.jpg)
![Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Western-Blot-NBP2-44470-img0005.jpg "Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (C21.48A1) followed by goat anti-Mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Immunocytochemistry-Immunofluorescence-NBP2-44470-img0004.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Flow Cytometric Analysis of PFA-fixed HeLa cells using beta 2-Microglobulin Antibody (C21.48A1)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Flow-Cytometry-NBP2-44470-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0014.jpg "Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0003.jpg)
![Western Blot: HLA DRA Antibody [NBP1-33458] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DRA antibody [N2C3] (NBP1-33458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-9102024201570.jpg)
![HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (NBP1-33458) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-immunocytochemistry-immunofluorescence-261120251821566.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0006.jpg "Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041]](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0002.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0001.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Isotype control. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP1-97041-img0008.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane 1: Non-reduced Rabbit IgG Isotype control. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Isotype control. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0007.jpg)