| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90054-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71420 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76871-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94913-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37276-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97521-0.025mg | |||
![Western Blot: CD44 Antibody [NBP3-14671] - Samples:Whole cell lysate (10 ug) from HeLa, 786-O, HEK293T,A-549, and KG-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD44 recombinant monoclonalantibody used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 3 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP3-14671-img0002.jpg "Western Blot: CD44 Antibody [NBP3-14671] - Samples:Whole cell lysate (10 ug) from HeLa, 786-O, HEK293T,A-549, and KG-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD44 recombinant monoclonalantibody used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 3 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody.")
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP3-14671] - FFPE section of head and neck squamous cell carcinoma. Rabbit anti-CD44 recombinant monoclonal antibody [BLR038F] (NBP3-14671 lot 1) used at 1:500. HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP3-14671-img0001.jpg)
![Western Blot: CD163 Antibody (OTI2B12) [NBP2-46115] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Western-Blot-NBP2-46115-img0003.jpg "Western Blot: CD163 Antibody (OTI2B12) [NBP2-46115] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.")
![Immunohistochemistry: CD163 Antibody (OTI2B12) [NBP2-46115] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Immunohistochemistry-NBP2-46115-img0002.jpg)
![Western Blot: CD163 Antibody (OTI2B12) [NBP2-46115] - Analysis of extracts (35ug) from 9 different cell lines by using CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Western-Blot-NBP2-46115-img0004.jpg)
![Immunofluorescence: CD163 Antibody (OTI2B12) [NBP2-46115] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Immunofluorescence-NBP2-46115-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR6 Antibody - BSA Free [NBP1-76871] - Immunofluorescence of CXCR6 in human spleen tissue with CXCR6 antibody at 20 ug/ml.Green: CXCR6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76871_rabbit-polyclonal-cxcr6-antibody-2711202419172291.gif "Immunocytochemistry/ Immunofluorescence: CXCR6 Antibody - BSA Free [NBP1-76871] - Immunofluorescence of CXCR6 in human spleen tissue with CXCR6 antibody at 20 ug/ml.Green: CXCR6 Antibody Blue: DAPI staining")
![Immunohistochemistry: CXCR6 Antibody [NBP1-76871] - Analysis of Bonzo in human spleen tissue with Bonzo antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR6-Antibody-Immunohistochemistry-NBP1-76871-img0006.jpg)
![Immunohistochemistry: CXCR6 Antibody - BSA Free [NBP1-76871] - Immunohistochemistry of CXCR6 in human spleen tissue with CXCR6 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76871_rabbit-polyclonal-cxcr6-antibody-2711202420414527.jpg)
![Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-Western-Blot-NBP1-51963-img0001.jpg "Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-Immunohistochemistry-Paraffin-NBP1-51963-img0002.jpg)
![Western Blot: RNF5 Antibody [NBP2-94913] - Analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/RNF5-Antibody-Western-Blot-NBP2-94913-img0004.jpg "Western Blot: RNF5 Antibody [NBP2-94913] - Analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Rat liver using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0003.jpg)
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Human liver cancer using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Mouse heart using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0002.jpg)
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-Western-Blot-NBP2-37276-img0003.jpg)
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BDNF-Antibody-3D9C5-ELISA-NBP2-37276-img0001.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0002.jpg "Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163.")
![Immunocytochemistry/Immunofluorescence: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD163.](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71420-img0004.jpg)
![Immunohistochemistry: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71420-img0003.jpg)
![Western Blot: CD163 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71420] - Analysis of extracts (35ug) from 9 different cell lines by using CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CD163-Antibody-OTI2B12-Azide-and-BSA-Free-Western-Blot-NBP2-71420-img0001.jpg)
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)