| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35076-1mg | |||
| Carrier-Free | NBP2-35076-100ug | |||
| Carrier-Free | NBP2-35076-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61863-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35252-1mg | |||
| Carrier-Free | NBP2-35252-100ug | |||
| Carrier-Free | NBP2-35252-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
![Western Blot: KIF3A Antibody (1C8K4) [NBP3-16657] - Western blot analysis of extracts of various cell lines, using KIF3A antibody (NBP3-16657) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Western-Blot-NBP3-16657-img0003.jpg "Western Blot: KIF3A Antibody (1C8K4) [NBP3-16657] - Western blot analysis of extracts of various cell lines, using KIF3A antibody (NBP3-16657) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit mAb (NBP3-16657) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Immunocytochemistry-Immunofluorescence-NBP3-16657-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunohistochemistry of paraffin-embedded rat testis using KIF3A Rabbit mAb (NBP3-16657) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KIF3A-Antibody-1C8K4-Immunohistochemistry-Paraffin-NBP3-16657-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: KIF3A Antibody (1C8K4) [NBP3-16657] - Immunofluorescence analysis of NIH-3T3 cells using KIF3A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16657_rabbit-kif3a-mab-1c8k4-52202511365138.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg)
![SDS-Page: Human TNF-alpha Protein [NBP2-35076]](http://images.novusbio.com/fullsize/Human-TNF-alpha-Protein-SDS-Page-NBP2-35076-img0001.jpg "SDS-Page: Human TNF-alpha Protein [NBP2-35076]")
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0005.jpg "Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Immunocytochemistry-Immunofluorescence-NBP2-61863-img0003.jpg)
![Flow Cytometry: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0002.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0004.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0006.jpg)
![ELISA: HLA DRA Antibody (1C11A5) [NBP2-61863] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![SDS-Page: Rat IL-4 Protein [NBP2-35252]](http://images.novusbio.com/fullsize/Rat-IL-4-Protein-SDS-Page-NBP2-35252-img0001.jpg "SDS-Page: Rat IL-4 Protein [NBP2-35252]")
![Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.](http://images.novusbio.com/fullsize/Recombinant-Rat-IL-4-Protein-Bioactivity-NBP2-35252-img0002.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442] - Staining (8ug/ml) of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunohistochemistry-Paraffin-NBP2-77442-img0004.jpg "Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442] - Staining (8ug/ml) of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.")
![Flow Cytometry: CXCR3 Antibody [NBP2-77442] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Flow-Cytometry-NBP2-77442-img0003.jpg)
![Immunohistochemistry-Paraffin: CXCR3 Antibody [NBP2-77442] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunohistochemistry-Paraffin-NBP2-77442-img0005.jpg)
![Immunofluorescence: CXCR3 Antibody [NBP2-77442] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunofluorescence-NBP2-77442-img0001.jpg)
![Immunofluorescence: CXCR3 Antibody [NBP2-77442] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunofluorescence-NBP2-77442-img0002.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Surface staining of human peripheral blood with anti-human CD108 (MEM-150) purified, GAM IgM PE.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0007.jpg "Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Surface staining of human peripheral blood with anti-human CD108 (MEM-150) purified, GAM IgM PE.")
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Analysis of FITC conjugate of NBP1-44896. Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0001.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Analysis of FITC conjugate of NBP1-44896. Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0002.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Analysis of FITC conjugate of NBP1-44896. Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0003.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Analysis of FITC conjugate of NBP1-44896. Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN HLA-A2:FITC](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0004.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Staining of human peripheral blood cells using anti-HLA-A2 (BB7.2) Alexa Fluor (R) 488.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0005.jpg)
![Flow Cytometry: HLA A2 Antibody (BB7.2) - Azide Free [NBP1-44896] - Staining of human peripheral blood cells using anti-HLA-A2 (BB7.2) PE.](http://images.novusbio.com/fullsize/HLA-A2-Antibody-BB7-2-Azide-Free-Flow-Cytometry-NBP1-44896-img0006.jpg)
![Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-288202412461675.jpg "Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29820241492635.jpg)
![Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-292024119237.jpg)
![Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29202416453215.jpg)
![Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-278202415295515.jpg "Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32209) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29820241435282.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-2920241192373.jpg)
![Flow Cytometry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Flow cytometric analysis of HepG2 cells labeling Complement C5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29202416372127.jpg)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0004.jpg "Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Human tonsil tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0003.jpg)
![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Analysis of paraffin-embedded human skin tissue using anti-IP10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0002.jpg)