| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43385-0.5mg | |||
| BSA Free | NBP1-43385-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94494-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74520 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80637 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79836 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64849SS | |||
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0002.jpg "Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0004.jpg)
![Flow Cytometry: PD-L1 Antibody [NB300-903] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Flow-Cytometry-NB300-903-img0005.jpg)
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed AA431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0003.jpg)
![Western Blot: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Western-Blot-NBP2-70364-img0001.jpg "Western Blot: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.")
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70364-img0002.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human pancreas tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0008.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0003.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0004.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0005.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human colon tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0006.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human liver tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0007.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0009.jpg)
![Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP3-14670-img0001.jpg "Western Blot: FoxP3 Antibody (BLR034F) [NBP3-14670] - Whole cell lysate (50 ug) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Rabbit anti-FOXP3 recombinant monoclonal antibody (NBP3-14670 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0004.jpg)
![Immunohistochemistry: FoxP3 Antibody (BLR034F) [NBP3-14670] - Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NBP3-14670-img0005.jpg)
![Immunoprecipitation: FoxP3 Antibody (BLR034F) [NBP3-14670] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXP3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. FOXP3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated FOXP3, was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunoprecipitation-NBP3-14670-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0007.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells")
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0001.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0004.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the FITC conjugate of NBP1-43385. Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 ug of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0005.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE conjugate of NBP1-43385. See Notes](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0006.jpg)
![Flow Cytometry: Armenian Hamster Monoclonal B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Alexa Fluor® 488 conjugated antibody (Catalog # NBP1-43385AF488) was tested via flow cytometry using magnetic beads coated with feline protein. Histogram overlay showing fluorescence results obtained via flow cytometry. Red line (unstained beads) and green line (beads coated with CD86 synthetic protein) served as negative controls. Blue line: positive signal obtained with CD80-coated beads. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-43385_armenian-hamster-monoclonal-b7-1-cd80-antibody-16-10a1-flow-cytometry-199202495917..png)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-1063-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0011.jpg)
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-1063-img0008.jpg)
![Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Flow-Cytometry-NB100-1063-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0010.jpg)
![Western Blot: SNRPD1 Antibody [NBP2-94494] - Western blot analysis of extracts of various cell lines, using SNRPD1 antibody (NBP2-94494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SNRPD1 Antibody-Western Blot-NBP2-94494-img0004.jpg "Western Blot: SNRPD1 Antibody [NBP2-94494] - Western blot analysis of extracts of various cell lines, using SNRPD1 antibody (NBP2-94494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: SNRPD1 Antibody [NBP2-94494] - Immunohistochemistry of paraffin-embedded rat kidney using SNRPD1 Rabbit pAb (NBP2-94494) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-94494-img0003.jpg)
![Immunohistochemistry-Paraffin: SNRPD1 Antibody [NBP2-94494] - Immunohistochemistry of paraffin-embedded human breast cancer using SNRPD1 Rabbit pAb (NBP2-94494) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-94494-img0001.jpg)
![Immunohistochemistry-Paraffin: SNRPD1 Antibody [NBP2-94494] - Immunohistochemistry of paraffin-embedded mouse kidney using SNRPD1 Rabbit pAb (NBP2-94494) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-94494-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: SNRPD1 Antibody - Azide and BSA Free [SNRPD1] - Immunofluorescence analysis of U2OS cells using SNRPD1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94494_rabbit-polyclonal-snrpd1-antibody-52202516283292.jpg)
![Immunocytochemistry/ Immunofluorescence: SNRPD1 Antibody - Azide and BSA Free [SNRPD1] - Immunofluorescence analysis of HeLa cells using SNRPD1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94494_rabbit-polyclonal-snrpd1-antibody-52202516345276.jpg)
![Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Western-Blot-NBP2-45933-img0009.jpg "Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)")
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0005.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0001.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0002.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0003.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0004.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0006.jpg)
![Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (PC61.5.3) [NB600-710] - Analysis of formalin-fixed paraffin-embedded tissue section of spleen from normal Mouse using CD25/IL-2 R alpha antibody (clone PC61.5.3.) at 1:100 dilution.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-PC61-5-3-Immunohistochemistry-Paraffin-NB600-710-img0001.jpg "Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (PC61.5.3) [NB600-710] - Analysis of formalin-fixed paraffin-embedded tissue section of spleen from normal Mouse using CD25/IL-2 R alpha antibody (clone PC61.5.3.) at 1:100 dilution.")
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0003.jpg "Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).")
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Adrenal stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0001.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0002.jpg)
![Protein Array: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIM-3 Antibody (TIM3/3113) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Protein-Array-NBP2-79702-img0004.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - TIM-3 was stained in human FFPE head and neck squamous cell carcinoma tissue. HIER antigen retrieval for 20min at pH 9. AF750 conjugated version of antibody was used (Catalog # NBP2-79836AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-79702_mouse-monoclonal-tim-3-antibody-tim3-3113-17720241044615.jpg)
![ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.](http://images.novusbio.com/fullsize/nb100-64849_mouse-monoclonal-ctla-4-antibody-bni3-2312025235988.jpg "ELISA: CTLA-4 Antibody (BNI3) [NB100-64849] - Human Anti-Abatacept Antibody bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated overnight with Mouse Anti-Human CD152 Antibody, clone BNI3 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of abatacept. Detection was performed using HRP conjugated Human Anti-Abatacept Antibody, clone AbD37058ia at a concentration of 2 µg/ml in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.")