| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50163-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23723SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11404 | |||
![ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP2-62853] - Samples were spiked with high concentrations of Mouse Insulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62853_mouse-insulin-elisa-kit-colorimetric-132202416243417.jpg "ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP2-62853] - Samples were spiked with high concentrations of Mouse Insulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Insulin ELISA Kit (Colorimetric) [NBP2-62853] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62853_mouse-insulin-elisa-kit-colorimetric-432024855098.jpg)
![Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0007.jpg "Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-77118-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-2711202419381793.jpg)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241933343.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM , (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77118_rabbit-polyclonal-bim-antibody-271120241928862.gif)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.")
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-40799-img0005.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0006.jpg)
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)](http://images.novusbio.com/fullsize/CD20-Antibody-L26-+-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44747-img0001.jpg "Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)")
![Immunohistochemistry-Paraffin: Latexin Antibody [NBP2-23723] - Analysis of Latexin in paraffin-embedded formalin-fixed rat brain tissue using an isotype control (top, left) and NBP2-23723 (bottom, left and right (100X)) at 1:100.](http://images.novusbio.com/fullsize/Latexin-Antibody-Immunohistochemistry-Paraffin-NBP2-23723-img0002.jpg "Immunohistochemistry-Paraffin: Latexin Antibody [NBP2-23723] - Analysis of Latexin in paraffin-embedded formalin-fixed rat brain tissue using an isotype control (top, left) and NBP2-23723 (bottom, left and right (100X)) at 1:100.")
![Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Western-Blot-NBP2-44470-img0005.jpg "Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (C21.48A1) followed by goat anti-Mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Immunocytochemistry-Immunofluorescence-NBP2-44470-img0004.jpg)
![Flow Cytometry: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Flow Cytometric Analysis of PFA-fixed HeLa cells using beta 2-Microglobulin Antibody (C21.48A1)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-C21-48A1-Flow-Cytometry-NBP2-44470-img0002.jpg)
![Western Blot: Dicer Antibody [NBP1-06521] - Whole cell lysate from 293T and HeLa cells. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06520 and another Dicer antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06521-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Dicer Antibody [NBP1-06521] - Whole cell lysate from 293T and HeLa cells. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06520 and another Dicer antibody.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0014.jpg "Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0003.jpg)
![Western Blot: HLA DRA Antibody [NBP1-33458] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DRA antibody [N2C3] (NBP1-33458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-9102024201570.jpg)
![HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (NBP1-33458) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-immunocytochemistry-immunofluorescence-261120251821566.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-NB100-57521-img0003.jpg)
![Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-SPM494-Flow-Cytometry-NBP2-44744-img0002.jpg "Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")