| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38450-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50226-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50443-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89738-50ul | |||
![Western Blot: Thrombopoietin R/Tpo R Antibody (5Q9Z6) [NBP3-15880] - Western blot analysis of extracts of various cell lines, using Thrombopoietin R/Tpo R Rabbit mAb (NBP3-15880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Thrombopoietin-R-Tpo-R-Antibody-5Q9Z6-Western-Blot-NBP3-15880-img0001.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (5Q9Z6) [NBP3-15880] - Western blot analysis of extracts of various cell lines, using Thrombopoietin R/Tpo R Rabbit mAb (NBP3-15880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Thrombopoietin R/Tpo R Antibody (5Q9Z6) [NBP3-15880] - Western blot analysis of extracts of various cell lines, using Thrombopoietin R/Tpo R Rabbit mAb (NBP3-15880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Thrombopoietin-R-Tpo-R-Antibody-5Q9Z6-Western-Blot-NBP3-15880-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody (6Q5C2) [NBP3-15392] - Immunohistochemistry of paraffin-embedded human appendix using Integrin alpha 2b/CD41 Rabbit mAb (NBP3-15392) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-6Q5C2-Immunohistochemistry-Paraffin-NBP3-15392-img0001.jpg "Immunohistochemistry-Paraffin: Integrin alpha 2b/CD41 Antibody (6Q5C2) [NBP3-15392] - Immunohistochemistry of paraffin-embedded human appendix using Integrin alpha 2b/CD41 Rabbit mAb (NBP3-15392) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot: C4 binding protein A Antibody [NBP3-38450] - Western blot analysis of lysates from Human plasma, using C4 binding protein A Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38450_rabbit-c4-binding-protein-a-pab-912202415121319.jpg "Western Blot: C4 binding protein A Antibody [NBP3-38450] - Western blot analysis of lysates from Human plasma, using C4 binding protein A Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: C4 binding protein A Antibody [NBP3-38450] - Western blot analysis of lysates from Mouse plasma, using C4 binding protein A Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38450_rabbit-c4-binding-protein-a-pab-912202415161886.jpg)
![Western Blot: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32468) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32468_rabbit-il-18-il-1f4-mab-ps00-41-278202416285231.jpg "Western Blot: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32468) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-18/IL-1F4 Antibody (PS00-41) [NBP3-32468] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32468) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32468) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32468_rabbit-il-18-il-1f4-mab-ps00-41-29820241358551.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Western-Blot-NBP2-89738-img0002.jpg "Western Blot: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:71 kDa")
![Immunoprecipitation: Serpin G1/C1 Inhibitor Antibody (18) [NBP2-89738] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-SERPING1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-SERPING1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 55 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-18-Immunoprecipitation-NBP2-89738-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Immunocytochemistry-Immunofluorescence-NBP2-89269-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269] - Analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Flow-Cytometry-NBP2-89269-img0001.jpg)
![Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-288202412493457.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-29202416372192.jpg)
![Western Blot: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32451) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32451_rabbit-il-18-il-1f4-mab-psh02-08-27820241633123.jpg "Western Blot: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Western blot analysis of IL-18/IL-1F4 on different lysates with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32451) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-18/IL-1F4 Antibody (PSH02-08) [NBP3-32451] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IL-18/IL-1F4 antibody (NBP3-32451) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32451) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32451_rabbit-il-18-il-1f4-mab-psh02-08-298202414161830.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")