| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99366-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12630-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99049-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89887-50ul | |||
![Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-29820241492673.jpg "Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: PD-L1 Antibody (PD01-02) [NBP3-32732] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate (low expression) Lane 5: HeLa cell lysate Lane 6: A549 cell lysate Lane 7: A549 treated with 100ng/mL IFN gamma for 48 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-3920248453543.jpg)
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0005.jpg "Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human spleen, lymphocytes.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0004.jpg)
![Western Blot: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Western blot analysis of Leukotriene B4 R1 on different lysates with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32529_rabbit-leukotriene-b4-r1-mab-je58-77-278202415295592.jpg "Western Blot: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Western blot analysis of Leukotriene B4 R1 on different lysates with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32529) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32529_rabbit-leukotriene-b4-r1-mab-je58-77-29820241433366.jpg)
![Western Blot: Plastin L Antibody [NBP2-99366] - Anti-Plastin L rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: Daudi Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Western-Blot-NBP2-99366-img0004.jpg "Western Blot: Plastin L Antibody [NBP2-99366] - Anti-Plastin L rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: Daudi Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Plastin L Antibody [NBP2-99366] - Plastin L was immunoprecipitated using: Lane A: 0.5 mg mouse lung tissue Lysate 4 ul anti-Plastin L rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Plastin L rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/Plastin-L-Antibody-Immunoprecipitation-NBP2-99366-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-99366] - Immunofluorescence staining of Plastin L in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99366 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99366_rabbit-polyclonal-plastin-l-antibody-235202511444468.png)
![Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) [NBP3-09151] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-NOK-1-Flow-Cytometry-NBP3-09151-img0002.jpg "Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) [NBP3-09151] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) [NBP3-09151] - Surface staining pattern of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Fas-Ligand-TNFSF6-Antibody-NOK-1-Flow-Cytometry-NBP3-09151-img0001.jpg)
![Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-278202415242680.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-298202414333652.jpg)
![Western Blot: CD41/CD61 Antibody (JM11-19) [NBP2-66757] - Analysis of CD41 on human placenta cells lysates using anti-CD41 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-JM11-19-Western-Blot-NBP2-66757-img0003.jpg "Western Blot: CD41/CD61 Antibody (JM11-19) [NBP2-66757] - Analysis of CD41 on human placenta cells lysates using anti-CD41 antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: CD41/CD61 Antibody (JM11-19) [NBP2-66757] - Analysis of paraffin-embedded human tonsil tissue using anti-CD41 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-JM11-19-Immunohistochemistry-Paraffin-NBP2-66757-img0002.jpg)
![Immunohistochemistry-Paraffin: CD41/CD61 Antibody (JM11-19) [NBP2-66757] - Analysis of paraffin-embedded human spleen tissue using anti-CD41 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD41-CD61-Antibody-JM11-19-Immunohistochemistry-Paraffin-NBP2-66757-img0001.jpg)
![Western Blot: DNAJC2 Antibody [NBP3-12630] - Lane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:71 kDa](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Western-Blot-NBP3-12630-img0001.jpg "Western Blot: DNAJC2 Antibody [NBP3-12630] - Lane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:71 kDa")
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP3-12630] - Staining of human DNAJC2 in human placenta with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP3-12630-img0003.jpg)
![Immunohistochemistry-Paraffin: DNAJC2 Antibody [NBP3-12630] - Staining of human DNAJC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DNAJC2-Antibody-Immunohistochemistry-Paraffin-NBP3-12630-img0002.jpg)
![Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-288202412493428.jpg "Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-29820241420920.jpg)
![Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-NBP2-99049-img0003.jpg "Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK alpha in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99049-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody [NBP2-99049] - Immunofluorescence staining of IKK alpha in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99049 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99049_rabbit-polyclonal-ikk-alpha-antibody-2352025166478.png)
![Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/Serum-Amyloid-A4-Antibody-001-Western-Blot-NBP2-89887-img0003.jpg "Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDa Observed band size:14 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Analysis of Human SAA4 expression on HepG2 cells. The cells were treated, stained with purified anti-Human SAA4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Serum-Amyloid-A4-Antibody-001-Flow-Cytometry-NBP2-89887-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Staining of Human SAA4 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89887 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89887_rabbit-monoclonal-serum-amyloid-a4-antibody-001-23520251664777.png)