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![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Western Blot: MARCO Antibody (PSH04-31) [NBP3-32568] - Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (NBP3-32568) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 53 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32568) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32568_rabbit-marco-mab-psh04-31-278202416331272.jpg "Western Blot: MARCO Antibody (PSH04-31) [NBP3-32568] - Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (NBP3-32568) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 53 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32568) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MARCO Antibody (PSH04-31) [NBP3-32568] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MARCO antibody (NBP3-32568) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32568) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32568_rabbit-marco-mab-psh04-31-298202414304115.jpg)
![Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-288202412442911.jpg "Western Blot: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/1,000 dilution. Lane 1: L-929 whole cell lysate Lane 2: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours whole cell lysate Lane 3: L-929 treated with pp for 1 hour whole cell lysate Lane 4: L-929 treated with 20uM Z-VAD for 3.5 hours, add 100nM SM-164 and 20ng/ml TNF-a for 3 hours, treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32795) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 [p Ser232] Antibody (JE44-62) [NBP3-32795] - Immunocytochemistry analysis of L-929 cells treated with or without 20μM Z-VAD for 2.75 hours, add 100nM SM-164 and 20ng/ml TNF-alpha for 2.25 hours, then treated with lambda pp for 1 hour labeling RIPK3/RIP3 with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32795) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32795_rabbit-ripk3-rip3-p-ser232-mab-je44-62-29202410234210.jpg)
![Flow Cytometry: NKp80/KLRF1 Antibody (5D12) [NBP3-14624] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/NKp80-KLRF1-Antibody-5D12-Flow-Cytometry-NBP3-14624-img0002.jpg "Flow Cytometry: NKp80/KLRF1 Antibody (5D12) [NBP3-14624] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).")
![Flow Cytometry: NKp80/KLRF1 Antibody (5D12) [NBP3-14624] - Separation of human NKp80 positive cells (red-filled) from human NKp80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/NKp80-KLRF1-Antibody-5D12-Flow-Cytometry-NBP3-14624-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NTAL Antibody (NAP-07) [NB500-341] - Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).](http://images.novusbio.com/fullsize/NTAL-Antibody-NAP-07-Immunocytochemistry-Immunofluorescence-NB500-341-img0001.jpg "Immunocytochemistry/Immunofluorescence: NTAL Antibody (NAP-07) [NB500-341] - Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).")
![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Western Blot: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Western blot analysis of PSMB10/MECL1 on different lysates with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN- for 48 hours cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Daudi cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: THP-1 cell lysate (20 ug/Lane) Lane 6: Ramos cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Jurkat cell lysate (20 ug/Lane) Lane 10: SiHa cell lysate (20 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32863) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32863_rabbit-psmb10-mecl1-mab-psh01-58-288202412442932.jpg "Western Blot: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Western blot analysis of PSMB10/MECL1 on different lysates with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN- for 48 hours cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Daudi cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: THP-1 cell lysate (20 ug/Lane) Lane 6: Ramos cell lysate (20 ug/Lane) Lane 7: U-937 cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Jurkat cell lysate (20 ug/Lane) Lane 10: SiHa cell lysate (20 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32863) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PSMB10/MECL1 Antibody (PSH01-58) [NBP3-32863] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10/MECL1 antibody (NBP3-32863) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32863_rabbit-psmb10-mecl1-mab-psh01-58-298202414161866.jpg)
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0002.jpg "Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.")
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0001.jpg)
![Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-278202415242688.jpg "Western Blot: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Western blot analysis of Complement Component C1qA on human kidney tissue lysates with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32097) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement Component C1qA Antibody (JE66-59) [NBP3-32097] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Complement Component C1qA antibody (NBP3-32097) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32097) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32097_rabbit-complement-component-c1qa-mab-je66-59-29820241411543.jpg)