| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99524-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12668-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90097-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99099-50ul | |||
![Western Blot: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32906) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32906_rabbit-ripk3-rip3-mab-psh04-79-288202412461630.jpg "Western Blot: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Western blot analysis of RIPK3/RIP3 on different lysates with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32906) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RIPK3/RIP3 Antibody (PSH04-79) [NBP3-32906] - Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIPK3/RIP3 antibody (NBP3-32906) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32906) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32906_rabbit-ripk3-rip3-mab-psh04-79-298202413585590.jpg)
![Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Immunocytochemistry-Immunofluorescence-NBP2-89269-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269] - Analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-028-Flow-Cytometry-NBP2-89269-img0001.jpg)
![Western Blot: Lysozyme Antibody (A5A8-R) [NBP3-32552] - Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (NBP3-32552) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: U-937 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32552_mouse-lysozyme-mab-a5a8-r-278202415242620.jpg "Western Blot: Lysozyme Antibody (A5A8-R) [NBP3-32552] - Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (NBP3-32552) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: U-937 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lysozyme Antibody (A5A8-R) [NBP3-32552] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (NBP3-32552) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32552) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32552_mouse-lysozyme-mab-a5a8-r-29820241358559.jpg)
![Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/FAT10-Antibody-Western-Blot-NBP2-99524-img0003.jpg "Western Blot: FAT10 Antibody [NBP2-99524] - Anti-FAT10 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 25 kDa")
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody [NBP2-99524] - Immunofluorescence staining of FAT10 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99524 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99524_rabbit-polyclonal-fat10-antibody-23520251628192.png)
![Western Blot: IFIT3 Antibody [NBP3-12668] - Lane A: HeLa Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:56 kDaObserved band size:58 kDa](http://images.novusbio.com/fullsize/IFIT3-Antibody-Western-Blot-NBP3-12668-img0001.jpg "Western Blot: IFIT3 Antibody [NBP3-12668] - Lane A: HeLa Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:56 kDaObserved band size:58 kDa")
![Immunoprecipitation: IFIT3 Antibody [NBP3-12668] - Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-IFIT3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IFIT3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 56 kDaObserved band size :58 kDa](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunoprecipitation-NBP3-12668-img0002.jpg)
![Western Blot: IFIT3 Antibody - BSA Free [NBP3-12668] - NBP3-12668 at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:58 kDa](http://images.novusbio.com/fullsize/nbp3-12668_rabbit-polyclonal-ifit3-antibody-235202511444453.png)
![Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-278202416331251.jpg "Western Blot: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Western blot analysis of LAMP-2/CD107b on different lysates with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32523) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAMP-2/CD107b Antibody (D3-D8-D3-R) [NBP3-32523] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP-2/CD107b antibody (NBP3-32523) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32523) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32523_mouse-lamp-2-cd107b-mab-d3-d8-d3-r-298202414304116.jpg)
![Immunocytochemistry: B220/CD45R Antibody (IVA103) [NB500-492] - Staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD45R (IVA103); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-IVA103-Immunocytochemistry-NB500-492-img0001.jpg "Immunocytochemistry: B220/CD45R Antibody (IVA103) [NB500-492] - Staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD45R (IVA103); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).")
![Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-288202412493457.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-29202416372192.jpg)
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0002.jpg "Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human appendix with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0001.jpg)
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Immunohistochemistry: HLA DP Antibody (PD00-70) [NBP3-32434] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DP antibody (NBP3-32434) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32434) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32434_rabbit-hla-dp-mab-pd00-70-29820241420989.jpg "Immunohistochemistry: HLA DP Antibody (PD00-70) [NBP3-32434] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DP antibody (NBP3-32434) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32434) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: HLA DP Antibody (PD00-70) [NBP3-32434] - Flow cytometric analysis of Daudi cells labeling HLA DP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32434_rabbit-hla-dp-mab-pd00-70-392024884637.jpg)
![Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NBP2-99099-img0002.jpg "Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99099] - Immunofluorescence staining of CHD4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99099 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99099_rabbit-polyclonal-chd4-antibody-235202514371324.png)