| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37265-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13550-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunocytochemistry-Immunofluorescence-NBP2-37265-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0004.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of bladder cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Flow cytometric analysis of Hela cells using CD68 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Flow-Cytometry-NBP2-37265-img0006.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against human CD68 (AA: 42-155) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against HEK293 (1) and CD68 (AA: 42-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0003.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of endometrial cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0007.jpg)
![ELISA: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-ELISA-NBP2-37265-img0001.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Western-Blot-NB120-13550-img0007.jpg "Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.")
![Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-017-0838-1), licensed under a CC-BY license](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13550-img0008.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human colon tumor tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0010.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human spleen tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0009.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg "Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.")
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Flow Cytometry: IFN-alpha 1 Antibody [NBP2-75930] - analysis of SH-SY5Y cells with IFNA1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Flow-Cytometry-NBP2-75930-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0003.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0005.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0006.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)