| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98729-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62562-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08990-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11967-0.025mg | |||
![Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-288202412493457.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-29202416372192.jpg)
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-2982024143520.jpg "Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Flow cytometric analysis of HEK-293 cells labeling B7-H3/CD276. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32138, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-29202416453234.jpg)
![Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-298202414161868.jpg "Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CD47 Antibody (PD00-33) [NBP3-32156] - Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32156, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-392024884610.jpg)
![Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-278202415295589.jpg "Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD79A Antibody (PDM0-23) [NBP3-32168] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79A antibody (NBP3-32168) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-29820241435290.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-98729] - Staining of mouse IRF6 in mouse small intestine with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP2-98729-img0002.jpg "Immunohistochemistry-Paraffin: IRF6 Antibody [NBP2-98729] - Staining of mouse IRF6 in mouse small intestine with rabbit polyclonal antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: IRF6 Antibody [NBP2-98729] - Immunofluorescence staining of IRF6 in COS7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98729 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98729_rabbit-polyclonal-irf6-antibody-235202521451760.png)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")
![Immunocytochemistry/Immunofluorescence: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Analysis of monoclonal antibody to SLAMF7 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-1B9-Immunocytochemistry-Immunofluorescence-H00057823-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Analysis of monoclonal antibody to SLAMF7 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Detection limit for recombinant GST tagged SLAMF7 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-1B9-Sandwich-ELISA-H00057823-M01-img0003.jpg)
![Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Immunohistochemistry-NBP2-62562-img0002.jpg "Immunohistochemistry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Immunohistochemical staining of human lung tissue using anti-CD55 antibody. LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (NBP2-62562). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62562 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD55/DAF Antibody (LU30) - Chimeric [NBP2-62562] - Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30. U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (NBP2-62562, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD55-DAF-Antibody-LU30-Chimeric-Flow-Cytometry-NBP2-62562-img0001.jpg)
![Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] - Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WASF2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23201_rabbit-wasf2-p-tyr150-pab-19920231410145.jpg "Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] - Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WASF2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).")
![Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-3D6-112-recombinant-version-Flow-Cytometry-NBP3-08990-img0001.jpg "Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.")
![Immunocytochemistry/Immunofluorescence: CD37 Antibody (MB-1) [NBP3-11967] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (NBP3-11967) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11967, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD37-Antibody-MB-1-Immunocytochemistry-Immunofluorescence-NBP3-11967-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD37 Antibody (MB-1) [NBP3-11967] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (NBP3-11967) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11967, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor(R) 488 secondary antibody.")