| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44921 | |||
| Azide and BSA Free | NBP2-34583-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43670-100ul | |||
| BSA Free | NBP3-43670-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65338-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12075-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26373-50ul | |||
![Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Western-Blot-NBP3-15612-img0004.jpg "Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0001.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: BTK Antibody (2J9O8) [BTK] - Immunofluorescence analysis of paraffin-embedded mouse spleen using BTK Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15612_rabbit-btk-mab-2j9o8-52202517311037.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - Formalin-fixed paraffin-embedded human tonsil stained with CD5 Monoclonal antibody (C5/473+CD5/54/F6)](http://images.novusbio.com/fullsize/CD5-Antibody-C5-473-+-CD5-54-F6-Immunohistochemistry-Paraffin-NBP2-34316-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - Formalin-fixed paraffin-embedded human tonsil stained with CD5 Monoclonal antibody (C5/473+CD5/54/F6)")
![Immunohistochemistry-Paraffin: CD5 Antibody (C5/473 + CD5/54/F6) [NBP2-34316] - CD5 CODEX stain of human tonsil tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34316_mouse-monoclonal-cd5-antibody-c5-473-cd5-54-f6-1772024936264.jpg)
![Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD20-Antibody-SPM494-Flow-Cytometry-NBP2-44744-img0002.jpg "Flow Cytometry: CD20 Antibody (SPM494) [NBP2-44744] - Flow Cytometric Analysis of Raji cells using CD20 Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)](http://images.novusbio.com/fullsize/CD20-Antibody-L26-+-IGEL-773-Immunohistochemistry-Paraffin-NBP2-44747-img0001.jpg "Immunohistochemistry-Paraffin: CD20 Antibody (L26 + IGEL/773) [NBP2-44747] - Human Lymphoma stained with CD20 Monoclonal Antibody (L26 + IGEL/773)")
![Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-[p-Tyr449]-Antibody-Western-Blot-NB110-85537-img0005.jpg "Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.")
![Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-[p-Tyr449]-Antibody-Western-Blot-NB110-85537-img0006.jpg)
![Western Blot of Rabbit aalpha/CD127 [p Tyr449] Antibody antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.](http://images.novusbio.com/fullsize/nb110-85537_rabbit-polyclonal-il-7r-alpha-cd127-p-tyr449-antibody-255202313135153.jpg)
![Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Western-Blot-NBP3-12916-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Rat lymph node. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse spleen. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0002.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse thymus gland. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0003.jpg)
![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0005.jpg "Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.")
![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0004.jpg)
![Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2682024158261.jpg "Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds")
![Western Blot: CD19 Antibody (PSH03-94) [NBP3-32130] - Western blot analysis of CD19 on mouse spleen tissue lysates with Rabbit anti-CD19 antibody (NBP3-32130) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32130) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-278202415212455.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD19 with Rabbit anti-CD19 antibody (NBP3-32130) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32130, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-29820241420976.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-CD19 antibody (NBP3-32130) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32130) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2982024149261.jpg)
![Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Western-Blot-NBP3-12075-img0001.jpg "Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12075-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12075_rabbit-monoclonal-cd43-sialophorin-antibody-84-3c1-chimeric-74202522141237.png)
![Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43,NBP2-32115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Western-Blot-NBP2-32115-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43,NBP2-32115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: CD43/Sialophorin Antibody [NBP2-32115] - Sample: FFPE section of human lung cancer infiltrating cells. Antibody: Affinity purified rabbit anti- CD43 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Immunohistochemistry-NBP2-32115-img0005.jpg)
![Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Western-Blot-NBP2-32115-img0001.jpg)
![Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-262202414471581.jpg)
![Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-2722024856312.jpg)
![Western Blot: BOB1 Antibody (4A5) [NBP3-26373] - Positive Western Blot detected in: Raji whole cell lysate. All lanes: BOB1 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26373_rabbit-bob1-mab-4a5-2822024115258.jpg)